We narrowed to 13,452 results for: Mpl;

1. pJOG250

   Plasmid

   #80582

   Purpose

   recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Inserts

   pnos:nptII-tnos
   p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
   BsaI-ccdB_CmR-BsaI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJOG251

   Plasmid

   #80583

   Purpose

   recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Inserts

   pnos:PAT-tnos
   p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
   BsaI-ccdB_CmR-BsaI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIG1_UL136_25kDa_Myc

   Plasmid

   #74954

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)

   Expression

   Mammalian

   Mutation

   delta 1-77, M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

   Plasmid

   #61796

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Ywhab 3'UTR with miR-200c MRE-1 mutated
   

   Use

   Luciferase

   Mutation

   miR-200c MRE-1 mutated

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

5. psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

   Plasmid

   #61793

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Xbp1 3'UTR with miR-200c MRE-3 mutated
   

   Use

   Luciferase

   Mutation

   miR-200c MRE-3 mutated

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

   Plasmid

   #61791

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Smad5 3'UTR with miR-200c MRE-1 mutated
   

   Use

   Luciferase

   Mutation

   miR-200c MRE-1 mutated

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

7. psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

   Plasmid

   #61790

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Map3k1 3'UTR with miR-200c MRE-1 mutated
   

   Use

   Luciferase

   Mutation

   miR-200c MRE-1 mutated

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

8. psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

   Plasmid

   #61789

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
   

   Use

   Luciferase

   Mutation

   both miR-200c MRE-2 and MRE-3 mutated

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

9. psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

   Plasmid

   #61786

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Crtap 3'UTR with miR-200c MRE-1 mutated
   

   Use

   Luciferase

   Mutation

   miR-200c MRE-1 mutated

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pSR11

    Plasmid

    #69154

    Purpose

    read-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    fzr-1 (fzr-1 Nematode)
    eGFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    fzr-1 and rps-27

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CFP(159-238) in pcDNAI/Amp

    Plasmid

    #60896

    Purpose

    This vector attaches CFP(159-238) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    CFP(159-238)
    

    Expression

    Mammalian

    Mutation

    Asn-164 to His

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT

    Plasmid

    #61787

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Fhod1 3'UTR with miR-200c MRE-2 mutated
    

    Use

    Luciferase

    Mutation

    miR-200c MRE-2 mutated

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. psiCHECK-2-Tob1-3'UTR-MRE-1 MUT

    Plasmid

    #61788

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Tob1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Mutation

    miR-200c MRE-1 mutated

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

14. psiCHECK-2-Zfp36-3'UTR-MRE-1 MUT

    Plasmid

    #61794

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Zfp36 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Zfp36 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Mutation

    miR-200c MRE-1 mutated

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

15. psiCHECK-2-Snail1-3'UTR-MRE-1 MUT

    Plasmid

    #61795

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Snail1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Snail1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Mutation

    miR-200c MRE-1 mutated

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

16. psiCHECK-2-Mapk12-3'UTR-MRE-1 MUT

    Plasmid

    #61797

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Mapk12 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Mapk12 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Mutation

    miR-200c MRE-1 mutated

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5 FRT TO myc Separase delta 55

    Plasmid

    #59824

    Purpose

    Allows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-N1-hRIP1-FL I541P

    Plasmid

    #61381

    Purpose

    Expresses Human full length RIPK1 with I541P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP1with I541P mutation (RIPK1 Human)
    

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    changed I541 to P

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. mKO-Alpha-Actinin-19

    Plasmid

    #57831

    Depositor

    Michael Davidson

    Article

    Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcdna3 T7 Jip1b(C631S, C643S and C699T)

    Plasmid

    #52128

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(M1-5) (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    C631S, C643S and C699T

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3 T7 Jip1b(471-707)

    Plasmid

    #52428

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-470

    Available Since

    April 29, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pcdna3 T7 Jip1b(282-707)

    Plasmid

    #52126

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(282-707)) (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-281

    Available Since

    April 18, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pCMV10/3xflag-Clock L113E

    Plasmid

    #47339

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L113E

    Promoter

    CMV

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pCMV10/3xflag-Clock F122D

    Plasmid

    #47340

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    F122D

    Promoter

    CMV

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pCDNA3-HA/BmalF423R

    Plasmid

    #47348

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    F423R

    Promoter

    CMV

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pEF5/FRT-DEST EVC2ala7-TEV-YFP-Flag

    Plasmid

    #41013

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1189-1192 to Alanine

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pEF5/FRT-DEST EVC2ala5-TEV-YFP-Flag

    Plasmid

    #41012

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1185-1188 to Alanine

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag

    Plasmid

    #41011

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1183-1186 to Alanine

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha

    Plasmid

    #33330

    Depositor

    Anthony Means

    Article

    Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

    Insert

    Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CaMKKbeta N-terminus (1-164) was fused to body fo…

    Promoter

    SV40

    Available Since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

30. HOXB6-FLAGA-pSP65

    Plasmid

    #20994

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    In vitro trans/trans

    Tags

    FLAG

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

31. psiCHECK GPR137B 3'UTR

    Plasmid

    #19864

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    GPR137B 3'UTR (GPR137B Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 5' Del 5

    Plasmid

    #16053

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter, 5' deletion (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -527 to +88 of promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

33. 5' Del 7

    Plasmid

    #16055

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter, 5' deletion (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -127 to +88 of promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

34. 5' Del 8

    Plasmid

    #16056

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter, 5' deletion (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -52 to +88 of promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

35. pET28a-MED11

    Plasmid

    #15414

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED11 (Med11 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

36. pNP3386 [For use in genome insertion and off-target assessment]

    Plasmid

    #247258

    Purpose

    Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRK5-HA-Ubiquitin-K48R

    Plasmid

    #17604

    Purpose

    Mammalian expression of HA tagged ubiquitin K48R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48R (expected to disrupt G76-K48 ubiquitin chain…

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pML104

    Plasmid

    #67638

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pXPR_051_dCas9-KRAB-HA

    Plasmid

    #228936

    Purpose

    All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28-MHL: nsp10_SARS-CoV-2

    Plasmid

    #167851

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp10

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. GST-Rab8a-QL

    Plasmid

    #86079

    Purpose

    A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. GST-Rab8a-TN

    Plasmid

    #86080

    Purpose

    A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed threonine 22 to aspargine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pIR-CMV-SCN2A-Variant-4-IRES-mScarlet

    Plasmid

    #209410

    Purpose

    Expression of wild-type neonatal isoform of human SCN2A.

    Depositor

    Al George

    Article

    Thompson et al bioRxiv. 2023 Feb 23:2023.02.23.529757.

    Insert

    SCN2A Variant 4, stabilized (SCN2A Human)
    

    Expression

    Mammalian

    Mutation

    IVS intron inserted after bp 4551 of the SCN2A OR…

    Promoter

    CMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pT7-hMLH1dn for IVT

    Plasmid

    #178114

    Purpose

    Template for in vitro transcription of human MLH1dn

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized) (MLH1 Human)
    

    Use

    Template for in vitro transcription

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pT7-EGFP-C1-HsDCP2-E148Q_U

    Plasmid

    #147650

    Purpose

    Mammalian Expression of HsDCP2-E148Q

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsDCP2-E148Q (DCP2 Human)
    

    Expression

    Mammalian

    Mutation

    two silent mutations compared to the sequence giv…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)

    Plasmid

    #206434

    Purpose

    Vasa 463-661 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Vasa 463-661 (vas Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Mutation

    F504E

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pJZC78

    Plasmid

    #62339

    Purpose

    sgRNA + 1x COM with COM-KRAB effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x COM binding module
    COM-KRAB
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

    Promoter

    CMV and U6

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pRK5-HA-Ubiquitin-K33

    Plasmid

    #17607

    Purpose

    Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K33 only, other lysines mutated to arginines; P19…

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pENTR221-JUN

    Plasmid

    #79512

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    JUN (JUN Human)
    

    Use

    Multisite gateway entry clone

    Tags

    HA Tag

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge