We narrowed to 8,219 results for: Lif

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pcDNA3-CKAR2

Plasmid

#123348

Purpose

Improved FRET-based biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

CKAR2

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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Nrp1-Fc-His

Plasmid

#72097

Purpose

Expresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp1 (Nrp1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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SspB-iRFP-DHPH-TIAM1

Plasmid

#176118

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Expression

Mammalian

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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Sema6a.a-Fc-His

Plasmid

#72163

Purpose

Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6a.a (Sema6a Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pKrox24(2xD-E)dTomato

Plasmid

#200110

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

modified EGR1 promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

2 copies of D-E element, no other promoter elemen…

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-tomato SOX2-sgRNA

Plasmid

#196190

Purpose

Editing human SOX2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

SOX2 (SOX2 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Plxna2-Fc-His

Plasmid

#72123

Purpose

Expresses the extracellular region of the PlexinA2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna2 (Plxna2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shQKI-3373

Plasmid

#115456

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shQKI-3373 (QKI Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAW-NbVHH05-mCherry

Plasmid

#171572

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-mCherry (UBE2J1 Human)

Tags

mCherry

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-mRuby3

Plasmid

#179727

Purpose

Lentiviral expression of SYP-mRuby3 for use as a synaptic vesicle marker

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYP (Syp Mouse)

Use

Lentiviral

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5A-FLAG

Plasmid

#72194

Purpose

Expresses full-length Unc5a with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5a (Unc5a Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA

Plasmid

#218870

Purpose

Mammalian expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2(no bind)

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

CAG

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBeta-lacZ-attBx2

Plasmid

#124445

Purpose

Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Type

Empty backbone

Use

Enhancer reporter assay construct

Tags

LacZ

Expression

Mammalian

Promoter

beta globin minimal promoter

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry C1 MFF

Plasmid

#157760

Purpose

Expression of mcherry:MFF in cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

MFF (MFF Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry-FKBP-TIAM1(DHPH)

Plasmid

#176138

Purpose

Heterodimerization with FRB (rapamycin), Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Lentiviral

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGY416

Plasmid

#166660

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

GAL1

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEntr1a-mcherry-FKBP-TIAM1(DHPH)

Plasmid

#176135

Purpose

Heterodimerization with FRB (rapamycin), Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Gateway Cloning

Mutation

See Depositor Comments Below

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJR107

Plasmid

#187245

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

Plasmid

#131683

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Promoter

EF1a and mU6

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEntr1a-SspB-HaloTag-TIAM1-DHPH

Plasmid

#176134

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Gateway Cloning

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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