We narrowed to 5,521 results for: crispr cas9 grna plasmid

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(517) SB KRAB-SadCas9 U6-gStop

Plasmid

#163023

Purpose

Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

Transposon

Tags

KRAB and myc NLS

Expression

Mammalian

Promoter

Tet-On

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

Plasmid

#223143

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 111-173

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1308-AAV-EFSNC-dSaCas9-HP1bNH(111-173)

Plasmid

#223153

Purpose

Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 111-173

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_LacZ_sgRNA

Plasmid

#155108

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_Luciferase_sgRNA

Plasmid

#155109

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting Luciferase

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

Luciferase_sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgSORCS2-G2

Plasmid

#118416

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA SORCS2 Human (SORCS2 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

Plasmid

#113702

Purpose

Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV, CRISPR, and Mouse Targeting

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

Plasmid

#113701

Purpose

Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

Plasmid

#192131

Purpose

Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_i1

Plasmid

#231416

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSaCas9-1xms2-2x3’UTR

Plasmid

#122946

Purpose

For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

SaCas9-MS2-HBB 3' UTR

Use

AAV

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSaCas9-1xPP7-2x3'UTR

Plasmid

#122947

Purpose

For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

SaCas9-PP7-HBB 3' UTR

Use

AAV

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EFS and hU6

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.tRFP

Plasmid

#57819

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

Plasmid

#195502

Purpose

Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA (SOX17 Human)

Use

CRISPR

Tags

HA

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9-KKH-Puro

Plasmid

#135966

Purpose

Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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CCND2 targeting gRNA

Plasmid

#215318

Purpose

Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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CCND1 targeting gRNA

Plasmid

#215153

Purpose

Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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MTF2_KO_ex11_gRNA-4

Plasmid

#131334

Purpose

gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

MTF2_KO_ex11_gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

Plasmid

#223164

Purpose

2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA2-gRNA_A

Plasmid

#74376

Purpose

gRNA_A to knockout human AMPK alpha 2 using Cas9n.

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)

Use

CRISPR

Promoter

U6

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA2-gRNA_B

Plasmid

#74377

Purpose

gRNA_B to knockout human AMPK alpha 2 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)

Use

CRISPR

Promoter

U6

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

Plasmid

#192133

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-KY

Use

AAV

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (K390A, Y446A)

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

Plasmid

#192132

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-Y

Use

AAV

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (Y446A)

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

Plasmid

#113699

Purpose

Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 RFP670

Plasmid

#187646

Purpose

lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site

Depositor

Martin Carroll

Insert

RFP 670

Use

Lentiviral

Tags

RFP670 and sgRNA cloning site

Promoter

EFS (P2A)

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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EED-KO-gRNA-2

Plasmid

#131322

Purpose

gRNA to knockout EED. Use with EED-KO-gRNA-1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-2

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

Plasmid

#232183

Purpose

Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6, hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pED9x (dCas9-KRAB-mCherry)

Plasmid

#163956

Purpose

Lentiviral expression plasmid of sgRNA with mCherry

Depositor

Junwei Shi

Article

Giles et al Immunity. 2022 Mar 8;55(3):557-574.e7. d

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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