We narrowed to 34,841 results for: cas

1. Lenti-EF1alpha-dCas9-KRAB_Hygro

   Plasmid

   #192663

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.minCMVe-SCP1-SaCas9-W3SynpA

   Plasmid

   #231370

   Purpose

   SCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   SaCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

   Plasmid

   #160611

   Purpose

   Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   P35s:LbCas12a:T35s
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCXLE-dCas9VP192-T2A-EGFP

   Plasmid

   #69536

   Purpose

   Episomal plasmid encoding dCas9VP192

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   dCas9-dCas9VP192-EGFP
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEX-SaCas9-U6-sgSlc17a8

   Plasmid

   #124848

   Purpose

   Mutagenesis of Slc17a8

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a8 (Slc17a8 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP185 pLVP-dCas9-DNMT3a V2

   Plasmid

   #100936

   Purpose

   dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLP339 pGK-dCas9-SunTag

   Plasmid

   #100943

   Purpose

   dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, SunTag array (10xGCN4)
   

   Use

   CRISPR

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dual-CRISPR-Cas9-U6-H1 (pBP48)

   Plasmid

   #218932

   Purpose

   The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   2A-Puro and 3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_UdgX-EE-UdgX-nCas9-UdgX

   Plasmid

   #163566

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2CT-His-MBP-Lba_Cas13a_WT

    Plasmid

    #91867

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbaCas13a (LbaC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFLAG-dCas9/pMXs-puro

    Plasmid

    #51240

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid

    #48226

    Purpose

    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    

    Use

    CRISPR

    Tags

    2A-puro, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-NRCH-ABE8.17-m+V106W

    Plasmid

    #198551

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-ABE8.17-m+V106W
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS79 (eft-3p::Cas9 + sgRNA)

    Plasmid

    #154839

    Purpose

    Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Anti-CASK [K56A/50R]

    Plasmid

    #177443

    Purpose

    Mammalian Expression Plasmid of anti-CASK (Human). Derived from hybridoma K56A/50.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-CASK (Homo sapiens) recombinant mouse monoclonal antibody (CASK Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis-MBP-BrCas12b-FNLDTA

    Plasmid

    #195340

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis Tag and MBP, TEV cleavage site, 6x His Tag

    Expression

    Bacterial

    Mutation

    F208W, N524V, L795I, D868V, T874S, A1015E

    Promoter

    T7 Promoter

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEX-SaCas9-U6-sgGabrg2

    Plasmid

    #124857

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX404 Campylobacter jejuni Cas9

    Plasmid

    #68338

    Purpose

    Campylobacter jejuni Cas9

    Depositor

    Feng Zhang

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUF-Cas9-pre-sgRNA

    Plasmid

    #137845

    Purpose

    Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_human-Caspase-4_enzymatic-domain

    Plasmid

    #183381

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (AA94-377) (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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