We narrowed to 14,350 results for: cas9
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Plasmid#100941PurposeSingle chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tagDepositorInsertscFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)
Tags1xHA and 3xTy1ExpressionMammalianMutationD10A, H840A for dCas9Available sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin
Plasmid#187954PurposeFKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-SpdCas9-hHDAC4-tagBFP
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
EC2_3_dCas9_Mxi1_sgRNA
Plasmid#163708PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressorDepositorInsertsdCas9
Mxi1+SV40 NLS
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSaGuide
Plasmid#64710PurposeCloning and expression of Staphylococcus aureus guide RNADepositorInsertStaphylococcus aureus guide RNA
UseCRISPRExpressionMammalianPromoterhU6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G1397 DddAtox-N–SaKKH-Cas9(D10A)
Plasmid#157839Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
ExpressionMammalianAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
L2_lacZgRNA-Cas9-CsA
Plasmid#136138PurposePlasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.DepositorInsertp5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSA072 Cas9-HBB_IVS2-BFP
Plasmid#249133PurposeOverexpression of SpCas9-BFP with HBB IVS2 intron after Cas9 coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.DepositorInsertCas9-HBB_IVS2-TagBFP (HBB S. pyogenes Cas9, synthetic, Human)
UseCRISPR; Cp36 donor dnaExpressionMammalianMutationHBB IVS2 intronPromoterEF1aAvailable sinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19-D1VXP4
Plasmid#103093PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertD1VXP4(Cas9 coding gene from Prevotella timonensis CRIS 5C-B1)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E0XXB7
Plasmid#103098PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE0XXB7(Cas9 coding gene from uncultured delta proteobacterium HF0070_07E19)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E7H313
Plasmid#103104PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE7H313(Cas9 coding gene from Sutterella wadsworthensis 3_1_45B)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST_119_LVL2 cam
Plasmid#179333PurposeNT-CRISPR plasmid for integration of multiple gRNAs.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
UseCRISPRAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCT310
Plasmid#111140PurposeBacterial expression of 6His-dCas9-3XFlagDepositorInsertdCas9
Tags3X Flag and 6X HisExpressionBacterialMutationD10A - H840APromoterT7Available sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)
Viral prep#112677-AAV1PurposeReady-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
hRad51(K133R)–Cas9(D10A)
Plasmid#125568PurposeExpresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJF152_Phsp_Cas9
Plasmid#163862Purposeheat shock-induced expression of Cas9 in C. elegansDepositorInsertCas9, C. elegans codon optimized with intron and SV40_NLS
UseCRISPRExpressionWormPromoterPhsp-16.48Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH0006
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianPromoterEF1AAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF1Cas-LT1
Plasmid#210556PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1pDepositorInsertpMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
ExpressionPlantAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-N22p-3XRFPnls
Plasmid#75387PurposeN22p-3XRFPnlsDepositorInsertN22p
UseLentiviralTags3XmCherryExpressionMammalianPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ABE9
Plasmid#194208PurposeMammalian expression of ABE9DepositorInsertTadA-8e (N108Q/L145T)-nSpCas9
UseCRISPRTags6*HisExpressionMammalianAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-mCherry-Cre-blast
Plasmid#179390PurposeLentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.DepositorInsertmCherry (P2A) Cre recombinase
UseCre/Lox and LentiviralExpressionMammalianPromoterCMVAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by