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p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA

Plasmid

#97010

Purpose

Rosa26 targeting vector for FLP-responsive cassette

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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nuc-yHS1

Plasmid

#159166

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1

Tags

SV40 NLS

Expression

Yeast

Promoter

GPD

Available Since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Tac-GFP

Plasmid

#162494

Purpose

Expresses Tac (IL2 recptor alpha subunit) with C-terminally tagged GFP in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

Tac (IL2RA Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pFETCh_CIC_iso1

Plasmid

#135728

Purpose

Donor vector for 3' FLAG tag of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 Homology arms (CIC Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Tac

Plasmid

#162489

Purpose

Expresses Tac (IL2 recptor alpha subunit) with GFP tag in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

Tac (IL2RA Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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mPlum-Gamma-Tubulin-17

Plasmid

#55971

Purpose

Localization: Centrosomes, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
TXNIP-py

Plasmid

#200090

Purpose

Dox-inducible human TXNIP with py motif mutations (ppcy,ppty -->paca,pata) in pInducer20 vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

TXNIP (TXNIP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

py motifs mutated (aa331-334 and 375-378)

Promoter

CMV

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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R26-EGFP MMEJ donor vector

Plasmid

#137926

Purpose

R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-EGFP-bpA with micro-homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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EYFP-Clathrin-19

Plasmid

#56584

Purpose

Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Clathrin (CLTB Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mVenus-CENPB-N-22

Plasmid

#56325

Purpose

Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

CENPB (CENPB Human)

Tags

mVenus

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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PX458_CIC_iso1_2

Plasmid

#135744

Purpose

Encodes gRNA for 3' target of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 gRNA (CIC Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_CIC_iso1_1

Plasmid

#135743

Purpose

Encodes gRNA for 3' target of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 gRNA (CIC Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRB

Plasmid

#193568

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-Fyn C3,6S

Plasmid

#19979

Depositor

Marilyn Resh

Article

Alland et al J Biol Chem. 1994 Jun 17. 269(24):16701-

Insert

Fyn (FYN Human)

Expression

Mammalian

Mutation

C3,6S

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag Rab1a N124I

Plasmid

#46778

Purpose

expression of N124I Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (Rab1a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N124I cannot stably bind guanine nucleotide

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
tdEos-Vimentin-N-18

Plasmid

#57690

Purpose

Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

NM_003380.3 (VIM Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Stx3-mCitrine-mCherry

Plasmid

#92426

Purpose

Expression of syntaxin-3 C-terminally conjugated to tandem of mCitrine and mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Tags

mCherry and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1α-spider-monkey-retroCHMP3-HA

Plasmid

#154179

Purpose

expresses spider monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1A3 EcoRI Methylase-AmilCP

Plasmid

#85166

Purpose

For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

Depositor

Robin Dowell

Article

Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

Insert

EcoRI Methylase-AmilCP

Tags

AmilCP

Expression

Bacterial

Promoter

J23100 promoter

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only
pIW086

Plasmid

#134132

Purpose

Bacterial expression of His-TEV-Ska1 and Ska2

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-TEV-human Ska1 ; human Ska2

Tags

His-TEV

Expression

Bacterial

Mutation

K183A K184A

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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