We narrowed to 32,675 results for: eng

1. EGFP-INPP4A

   Plasmid

   #171944

   Purpose

   Expresses INPP4A in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   INPP4A (INPP4A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-U6a-U6c-ndufs2 guide

   Plasmid

   #107601

   Purpose

   An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   ndufs2 guide RNA sequences
   

   Use

   CRISPR

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK2301

   Plasmid

   #65778

   Purpose

   Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   St1Cas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTK402

   Plasmid

   #110598

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. iCAP-BI155-KanR

   Plasmid

   #209527

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

   Insert

   AAV-BI155 Cap
   

   Expression

   Mammalian

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fyn-Fyn Biosensor

   Plasmid

   #124655

   Purpose

   FRET biosensor. Fyn-tagged biosensor targeting to membrane

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

   Insert

   Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP977

   Plasmid

   #65774

   Purpose

   Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135E mutation in Cas9

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMBA333

   Plasmid

   #214745

   Purpose

   Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3
   

   Expression

   Bacterial

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-U6a-U6c-rac2 guide

   Plasmid

   #107600

   Purpose

   An entry vector with U6a and U6c promoter driving rac2 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   rac2 guide RNA sequences
   

   Use

   CRISPR

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTK501

    Plasmid

    #110602

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBTK229

    Plasmid

    #110591

    Purpose

    BTK Type 3 coding sequence for golden gate assembly and homologous recombination

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Kanamycin resistance
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pBTK570

    Plasmid

    #110615

    Purpose

    BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    E2-Crimson
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pBTK520

    Plasmid

    #110604

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pBTK510

    Plasmid

    #110611

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV-CMV-mTurquoise2-NLS

    Plasmid

    #165443

    Purpose

    Expresses nuclear mTurquoise2 from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    mTurquoise2-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBTK564

    Plasmid

    #110613

    Purpose

    BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Nanoluc
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pBTK599

    Plasmid

    #110600

    Purpose

    BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP dropout
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pSUPERIORpuro-shGAS5

    Plasmid

    #46370

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

    Insert

    GAS5 (GAS5 Human)
    

    Use

    RNAi

    Promoter

    H1

    Available Since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

19. Ensconsin-18-mEos2

    Plasmid

    #99230

    Purpose

    Mammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    mEos2
    

    Tags

    Ensconsin (MAP7)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ262m3

    Plasmid

    #149556

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, R221K, N394K, Protospacer interacting domai…

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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