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midSIIN

Plasmid

#185665

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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LV-PGK-Cre-EFS-mScarletSIIN

Plasmid

#172434

Purpose

Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

Lentiviral

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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H11-mScarletSIIN

Plasmid

#172438

Purpose

Hipp11 targeting vector, expresses mScarlet-SIINFEKL

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-SIIN

Use

Mouse Targeting

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGAL4DBD-SuUR f.l

Plasmid

#112995

Purpose

P element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Sher et al Genome Res. 2012 Jan;22(1):64-75. doi: 1

Insert

SuUR (SuUR Fly)

Use

P insertion vector

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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noSIIN

Plasmid

#185666

Purpose

shApc-expressing lentiviruses with no expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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U6-sgfiller-eCas9-T2A-BlastR

Plasmid

#172437

Purpose

Expresses eCas9, BlastR and sgRNA cloning site

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

eCas9
BlastR

Use

CRISPR, Lentiviral, and Mouse Targeting

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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H11-mScarletALG8

Plasmid

#172440

Purpose

Hipp11 targeting vector, expresses mScarlet-mALG8

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-mALG8

Use

Mouse Targeting

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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R26-dCas9-VPR-mNG

Plasmid

#172441

Purpose

Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

dCas9-VPR
mNeonGreen

Use

Mouse Targeting

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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U6-sgR26-eCas9-T2A-BlastR

Plasmid

#172493

Purpose

For CRISPR-assisted HDR, Rosa26 locus

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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CYLBL_Neo_CAGdCas9-KRAB

Plasmid

#220439

Purpose

CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

Depositor

Yin Shen

Article

Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

Insert

dCas9

Use

CRISPR and TALEN

Tags

KRAB

Expression

Mammalian

Mutation

D10A and H840A

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

Plasmid

#172436

Purpose

Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

CRISPR and Lentiviral

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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WT_IFL_G1A-T2A_R

Plasmid

#200183

Purpose

WT PA14 I-F Leader and G1A-T2A Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A)

Expression

Bacterial

Mutation

G1A-T2A in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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WT_IFL_A28C_R

Plasmid

#200184

Purpose

WT PA14 I-F Leader and A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (A28C)

Expression

Bacterial

Mutation

A28C in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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WT_IFL_G1A-T2A-A28C_R

Plasmid

#200185

Purpose

WT PA14 I-F Leader and G1A-T2A-A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)

Expression

Bacterial

Mutation

G1A-T2A-A28C in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-457

Plasmid

#200213

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 H25A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-458

Plasmid

#200214

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 E184A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-459

Plasmid

#200215

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 K214A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-460

Plasmid

#200216

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas2 R55E-N56D mutations

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-461

Plasmid

#200217

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas2 K11D-R12E mutations

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-462

Plasmid

#200218

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas2 K11D-R12E, R55E-N56D mutations

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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