163,743 results

1. pYMA3_SidK_GFP

   Plasmid

   #184276

   Purpose

   Legionella pneumophila SidK (1-278) - GFP

   Depositor

   John Rubinstein

   Article

   Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

   Insert

   SidK (1-278) (sidK Legionella pneumophila)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP (AAV Retrograde)

   Viral Prep

   #55639-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO hChR2(H134R)-EYFP (#55639). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO hChR2(H134R)-EYFP plasmid DNA. Ef1a-driven, Flp-dependent expression of ChR2(H134R)-EYFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a

   Tags

   EYFP

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

3. TRUPATH Triple Gai1

   Plasmid

   #196048

   Purpose

   Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai1-RLuc8 (GNAI1 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.2_mCherry_mito Grx1-roGFP2

   Plasmid

   #155044

   Purpose

   Fluorescent redox reporter

   Depositor

   Vanessa LaPointe

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Lentiviral

   Tags

   mitochondrial targeting sequence and roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH2.22_mCherry-MS2x8

   Plasmid

   #205537

   Purpose

   Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. human beta-catenin pcDNA3

   Plasmid

   #16828

   Purpose

   Mammalian expression of human beta-catenin with Flag tag

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-HA-NONO

   Plasmid

   #127655

   Purpose

   Over-express HA-tagged human NONO

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

   Insert

   NONO (NONO Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-TRIM28

   Plasmid

   #124960

   Purpose

   Mammalian expression of Human TRIM28

   Depositor

   Michelle Barton

   Article

   Li et al Oncogene. 2017 May 25;36(21):2991-3001.

   Insert

   hTRIM28 (TRIM28 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV8)

   Viral Prep

   #105540-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   Syn

   Tags

   EGFP

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pDONR223-JAK2

    Plasmid

    #23915

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    JAK2 (JAK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. PGK mScarlet-Synaptophysin

    Plasmid

    #206145

    Purpose

    PGK mScarlet-Synaptophysin

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    synaptophysin (Syp Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmcherry α-catenin WT

    Plasmid

    #178646

    Purpose

    express wild-type α-catenin

    Depositor

    Daniel Conway

    Article

    Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

    Insert

    α-catenin (CTNNA1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV_3xFLAG-ATP6V0A3-mScarlet

    Plasmid

    #228867

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmiRFP720-N1

    Plasmid

    #136560

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP720
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFGAMS-mCherry

    Plasmid

    #166024

    Purpose

    Expresses human PFAS-mCherry fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    Doigneaux et al J Biol Chem. 2020 Jul 10;295(28):9551-95

    Insert

    PFAS-mCherry (PFAS Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lenti-EF1a-dCas9-KRAB-Puro

    Plasmid

    #99372

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-KRAB-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

    Plasmid

    #182926

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Use

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 (AAV9)

    Viral Prep

    #164510-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 (#164510). In addition to the viral particles, you will also receive purified pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 plasmid DNA. Synapsin-driven, cytosolic-targeted iGlucoSnFR-mRuby2

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Promoter

    Syn

    Tags

    mRuby2

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

19. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209725

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. STIM1-EGFP

    Plasmid

    #210370

    Purpose

    Expresses STIM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge