We narrowed to 1,031 results for: hug;

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pLenti-Cas9-GFP

Plasmid

#86145

Purpose

Lentiviral vector expressing Cas9-GFP

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

Cas9-GFP

Use

CRISPR and Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-Cas9

Plasmid

#242759

Purpose

Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Article

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-Cas9

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST H2B-mCerulean3

Plasmid

#90234

Purpose

Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST H2B-mRuby2

Plasmid

#90236

Purpose

Lentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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p-dCas9-SSAP-MS2-BB_BbsI

Plasmid

#183826

Purpose

pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

U6-guideRNA-dCas9-T2A-EBFP

Use

CRISPR

Expression

Mammalian

Promoter

CBH

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR

Plasmid

#118409

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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GA-NES

Plasmid

#61018

Purpose

ddGFP A-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev

Plasmid

#118474

Purpose

ddRFP-based single-color biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2

Plasmid

#28243

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR(T/A)

Plasmid

#118410

Purpose

Negative-control mutant for ExRai-CKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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CIB1-CreC(N1)

Plasmid

#75367

Purpose

Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Cre DNA recombinase, residues 106-343

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-SN

Plasmid

#181741

Purpose

AAV vector for expressing SN

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

SuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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GB-NES

Plasmid

#61017

Purpose

ddGFP B-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP B

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-DDX3X

Plasmid

#70648

Purpose

Expresses WT DDX3X in mammalian cells under blasticidin selection.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

DDX3X (DDX3X Human)

Use

Retroviral

Tags

3x FLAG

Expression

Mammalian

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3 Leo1

Plasmid

#11061

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Leo1 (LEO1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2PHR

Plasmid

#28244

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

cry2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Available Since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-C16orf80-flag

Plasmid

#70645

Purpose

Expresses C16orf80 in mammalian cells with FLAG-tag epitope.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C16orf80 (CFAP20 Human)

Use

Retroviral

Tags

3X FLAG

Expression

Mammalian

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AktAR

Plasmid

#118462

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pR-mTagBFP2

Plasmid

#228596

Purpose

Mammalian expression of mTagBFP2, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mTagBFP2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mRuby3

Plasmid

#228609

Purpose

Mammalian expression of mRuby3, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mRuby3

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sapphireCKAR

Plasmid

#118468

Purpose

cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireCKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Paf1

Plasmid

#11059

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Paf1 (PAF1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pUCHR-osTIR1-IRES-GFP

Plasmid

#110661

Purpose

Lentiviral vector for stable expression of osTIR1 and selection with GFP

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

osTIR1

Use

Lentiviral

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-AKARev

Plasmid

#118478

Purpose

ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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CIB81-pmGFP

Plasmid

#75366

Purpose

Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

prenylated EGFP

Expression

Mammalian

Mutation

residues 1-81 of CIB1

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pREDIT_MCP-RecT

Plasmid

#164803

Purpose

mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Insert

RecT

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pR-mKate2

Plasmid

#228610

Purpose

Mammalian expression of mKate2, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mKate2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCIB1(deltaNLS)-pmGFP

Plasmid

#28240

Purpose

Expresses CIB1 fused to plasma membrane-localized EGFP

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pNluc-hbb

Plasmid

#239753

Purpose

Template for in vitro transcription of nanoluciferase flanked by the human haemoglobin beta 5' and 3' UTRs.

Depositor

Catherine Jopling

Article

Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

Insert

Nanoluciferase flanked by human haemoglobin beta 5' and 3' UTRs

Use

Luciferase

Promoter

T7 (included as part of insert)

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Promoter

opposing T7 promoters

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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