We narrowed to 7,431 results for: Pet2

1. pET21-Drp1

   Plasmid

   #72927

   Purpose

   Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site

   Depositor

   David Chan

   Article

   Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

   Insert

   Drp1 (Dnm1l Mouse)
   

   Use

   Tags

   6xHis-tag and PreScission Protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21 E2F1

   Plasmid

   #90313

   Purpose

   Expresses human E2F1 in bacteria

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F1 (E2F1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET24c(+)-mtEFTu

   Plasmid

   #30535

   Depositor

   Linda Spremulli

   Article

   Woriax et al Biochim Biophys Acta. 1995 Dec 27. 1264(

   Insert

   Mature bovine mitochondrial elongation factor Tu
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Mature form amino acids 44-452. Mito import seque…

   Promoter

   Available since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pET22b-ecDHFR

   Plasmid

   #109055

   Purpose

   Bacterial expression of E.coli DHFR protein

   Depositor

   Stephen Benkovic

   Article

   Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

   Insert

   ecDHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Human _TFAM_NoMTS_Dimer_pET28a+

   Plasmid

   #60013

   Purpose

   Expresses TFAM No MTS dimerization mutant in bacterial cells

   Depositor

   David Chan

   Article

   Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

   Insert

   Human TFAM No MTS (TFAM Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Missing 1st 42 aas (Mitochondrial targeting seque…

   Promoter

   T7

   Available since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a(ICOS)

   Plasmid

   #177903

   Purpose

   Bacterial expression of his10-tagged human ICOS Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   ICOS (aa 162-199 only) (ICOS Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-C9ORF80

   Plasmid

   #128418

   Purpose

   Express C9ORF80 in E. coli with a His tag (C-terminal)

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

   Insert

   C9ORF80 (INIP Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-ec_bPAC

   Plasmid

   #28135

   Purpose

   Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   E.coli adapted photoactivated adenylyl cyclase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET22b-T5-mad32TAG

   Plasmid

   #188997

   Purpose

   Express madanin-1 with TAG at 32 position

   Depositor

   Han Xiao

   Article

   Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

   Insert

   madanin-1 with TAG at 32 position
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET22b-T5-mad

    Plasmid

    #188998

    Purpose

    Express wildtype madanin-1

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Insert

    wildtype madanin-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28b::mAID

    Plasmid

    #15923

    Depositor

    Nina Papavasiliou

    Article

    Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

    Insert

    AID (Aicda Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET22b-EC12WTC9AAvi

    Plasmid

    #47925

    Purpose

    Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Use

    Tags

    6xHis, Avi Tag, and TEV Cleavage

    Expression

    Bacterial

    Mutation

    changed Cysteine 9 to Alanine

    Promoter

    T7

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21c(+)-EFG1mt

    Plasmid

    #31076

    Depositor

    Linda Spremulli

    Article

    Bhargava et al Protein Expr Purif. 2004 Oct . 37(2):368

    Insert

    Mature form human mitochondrial elongation factor G isoform 1 (GFM1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes the coding sequence for the mature form.…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pET21c(+)-mtPheRS

    Plasmid

    #31080

    Depositor

    Linda Spremulli

    Article

    Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

    Insert

    Mature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes coding sequence for the mature form. Nt …

    Promoter

    T7

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pET28C-tCorn

    Plasmid

    #124588

    Purpose

    Bacterial Expression of tRNA-Corn aptamer

    Depositor

    Samie Jaffrey

    Article

    Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

    Insert

    tRNA-Corn
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET22b-EC12WTC9A

    Plasmid

    #47924

    Purpose

    Expresses E-cadherin EC12 WT with C9A mutation in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Use

    Tags

    6xHis and TEV Cleavage

    Expression

    Bacterial

    Mutation

    changed Cysteine 9 to Alanine

    Promoter

    T7

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-PSAT

    Plasmid

    #74115

    Purpose

    Expression of PSAT

    Depositor

    Lewis Cantley

    Article

    Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    pET28a-PSAT (PSAT1 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-MinC

    Plasmid

    #133628

    Purpose

    Expression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Loose et al Nat Struct Mol Biol. 2011 May;18(5):577-

    Insert

    His-MinC (minC E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28_6xHis-PARP1delZn3

    Plasmid

    #173936

    Purpose

    Bacterial expression of PARP1 lacking Zn3 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn3 (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 214-373; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28-MRPP1

    Plasmid

    #67862

    Purpose

    bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP1 (TRMT10C Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    mitoch. form (contains aa 40-403)

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a(cd226)

    Plasmid

    #177900

    Purpose

    Bacterial expression of his10-tagged human cd226 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    CD226 (aa 288-336 only) (CD226 Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28-OmpT

    Plasmid

    #68862

    Purpose

    construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

    Depositor

    Neil Kelleher

    Article

    Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

    Insert

    OmpT (ompT )
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-Beclin1 CC

    Plasmid

    #24403

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains the CC domain (aa 151-242)

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pET28-HADH2

    Plasmid

    #67863

    Purpose

    bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    HADH2 (HSD17B10 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28b-PaODH

    Plasmid

    #110802

    Purpose

    Expresses PaODH and opine dehydrogenase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

    Insert

    Pa4835
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28b-PaNAS

    Plasmid

    #110801

    Purpose

    Expresses PaNAS nicotianamine synthase from pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

    Insert

    Pa4836
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET23a-HsUBB

    Plasmid

    #69562

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pET28b-Ava3855

    Plasmid

    #45743

    Depositor

    Christopher T Walsh, Emily Balskus

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3855 (Ava_3855 Anabaena variabilis)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pET29b-EPN-01*

    Plasmid

    #80423

    Purpose

    For expression of EPN-01* in E. coli

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01*
    

    Use

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

30. pET28a(TIGIT)

    Plasmid

    #177902

    Purpose

    Bacterial expression of his10-tagged human TIGIT Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    TIGIT (aa 163-244 only) (TIGIT Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET26b(+)-ATI_QconCAT_1

    Plasmid

    #163957

    Purpose

    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

    Depositor

    Stefan Tenzer

    Article

    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

    Insert

    ATI QconCAT
    

    Use

    Tags

    polyhistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28-mTDGa.0

    Plasmid

    #81050

    Purpose

    bacterial expression of murine TDG

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET23a RanGAP

    Plasmid

    #13387

    Depositor

    Mary Dasso

    Article

    Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

    Insert

    RanGAP (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pET23b-proMMP-7

    Plasmid

    #29524

    Depositor

    William Parks

    Article

    Ra et al J Biol Chem. 2009 Oct 9. 284(41):27924-3

    Insert

    proMMP-7 (MMP7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pET28a-R4-uAb

    Plasmid

    #101801

    Purpose

    Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

    Depositor

    Matthew DeLisa

    Article

    Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

    Insert

    R4-uAb
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28_6xHis-PARP1delZn1

    Plasmid

    #173933

    Purpose

    Bacterial expression of PARP1 lacking Zn1 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn1 (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 1-96; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28_6xHis-PARP1-HD

    Plasmid

    #174809

    Purpose

    Bacterial expression of isolated PARP1 HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-HD (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21a - SodA

    Plasmid

    #89612

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase A
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET22-HT/plsA

    Plasmid

    #73625

    Purpose

    Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

    Depositor

    T. J. Kappock, James Williamson

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28-Kss1

    Plasmid

    #52682

    Purpose

    Expression of Kss1 in bacteria

    Depositor

    Benjamin Turk

    Article

    Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

    Insert

    KSS1 (KSS1 Budding Yeast)
    

    Use

    Tags

    His6 and T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only