Skip to main content
Addgene
Showing: 521 - 560 of 1185 results

  3. pCAG-hPPP3R2

    Plasmid
    #179163
    Purpose
    Expression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do
    Insert
    protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179163-plasmid-map-sequence-id-352820

    Enlarge

  4. pCAG-mPpp3r2

    Plasmid
    #179161
    Purpose
    Expression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2), CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do
    Insert
    protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179161-plasmid-map-sequence-id-352819

    Enlarge

  7. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159501
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159501-plasmid-map-sequence-id-311777

    Enlarge

  8. pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159502
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(4)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159502-plasmid-map-sequence-id-311727

    Enlarge

  9. pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159504
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(1)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159504-plasmid-map-sequence-id-311728

    Enlarge

  10. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159506
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159506-plasmid-map-sequence-id-311732

    Enlarge

  11. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159508
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(0)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159508-plasmid-map-sequence-id-311756

    Enlarge

  12. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(16)nts_Tornado-Corn

    Plasmid
    #159511
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 16 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(16)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159511-plasmid-map-sequence-id-311774

    Enlarge

  13. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(19)nts_Tornado-Corn

    Plasmid
    #159514
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 19 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(19)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159514-plasmid-map-sequence-id-311759

    Enlarge

  14. pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(20)nts_Tornado-Corn

    Plasmid
    #159515
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 20 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-3_PolyU(4)_HairpinDistance(20)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159515-plasmid-map-sequence-id-311758

    Enlarge

  15. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159499
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-2_PolyU(1)_HairpinDistance(0)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159499-plasmid-map-sequence-id-311745

    Enlarge

  16. pAV-U6+27-Linear-1_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid
    #159498
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(4)_HairpinDistance(10)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159498-plasmid-map-sequence-id-311780

    Enlarge

  17. pAV-U6+27-Linear-1_PolyU(4) _HairpinDistance(0)_Tornado-Corn

    Plasmid
    #159496
    Purpose
    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(4) _HairpinDistance(0)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159496-plasmid-map-sequence-id-311762

    Enlarge

  18. pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn

    Plasmid
    #159495
    Purpose
    Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination Module:Linear-1_PolyU(1)_HairpinDistance(0)nts
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6-27
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159495-plasmid-map-sequence-id-311767

    Enlarge

  19. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid
    #170186
    Purpose
    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing
    Depositor
    Bradley Bernstein
    Article
    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:
    Insert
    CreERT2-T2A-eBFP2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170186-plasmid-map-sequence-id-336799

    Enlarge

  22. pCAG-hPPP3CC-FLAG

    Plasmid
    #179162
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do
    Insert
    protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179162-plasmid-map-sequence-id-352821

    Enlarge

  24. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid
    #99692
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    99692-plasmid-map-sequence-id-194594

    Enlarge

  30. pCAG-hPPP3CB-FLAG

    Plasmid
    #179135
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179135-plasmid-map-sequence-id-352807

    Enlarge

  38. pCAG-hPPP3R1

    Plasmid
    #179136
    Purpose
    Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179136-plasmid-map-sequence-id-352789

    Enlarge

Showing: 521 - 560 of 1185 results