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Tau BA 12->7 G272V

Plasmid

#203368

Purpose

For transfection and in vitro assay

Depositor

Stefan Stamm

Article

Margvelani et al Biochim Biophys Acta Mol Basis Dis. 2024

Insert

MAPT (MAPT Human)

Expression

Mammalian

Mutation

G272V

Promoter

CMVf

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pPMS-2272

Plasmid

#232292

Purpose

Protein Expression

Depositor

Jeremy Nathans

Insert

Alpha bungarotoxin

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pPMS-2272

Plasmid

#232292

Purpose

Protein Expression

Depositor

Jeremy Nathans

Insert

Alpha bungarotoxin

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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CILK1-R272Q

Plasmid

#214911

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Tags

Myc-FLAG

Expression

Mammalian

Mutation

R272Q substitution

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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CILK1-R272Q

Plasmid

#214911

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Tags

Myc-FLAG

Expression

Mammalian

Mutation

R272Q substitution

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr272

Plasmid

#136755

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr272

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr272

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr272

Plasmid

#136755

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr272

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr272

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133272

Plasmid

#152459

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp9

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;CBP

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133272

Plasmid

#152459

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp9

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;CBP

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046272

Plasmid

#145421

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp9

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046272

Plasmid

#145421

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp9

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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YCe2721 HC_Kan_conn_W-Z

Plasmid

#100664

Purpose

EMMA toolkit Assembly connector W-Z

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector W-Z

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2721 HC_Kan_conn_W-Z

Plasmid

#100664

Purpose

EMMA toolkit Assembly connector W-Z

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector W-Z

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG272-eya

Plasmid

#99085

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea eyes absent (eya)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

eyes absent (eya)

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG272-eya

Plasmid

#99085

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea eyes absent (eya)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

eyes absent (eya)

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_puro-mKate-lox2272

Plasmid

#162076

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_puro-mKate-lox2272

Plasmid

#162076

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_hygro-tagBFP-lox2272

Plasmid

#162078

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_hygro-tagBFP-lox2272

Plasmid

#162078

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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CDK1 gRNA (BRDN0001149272)

Plasmid

#77390

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK1 (CDK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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