167,468 results

1. HCT47_pAAV-eHGT_3Xcore_1137m-minBGprom-iCre297T-WPRE3-bGHpA

   Plasmid

   #233564

   Purpose

   Direct-expressing SYFP2 AAV virus

   Depositor

   Allen Institute for Brain Science, Tanya Daigle

   Article

   Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

   Insert

   SYFP2
   

   Use

   AAV

   Promoter

   minBglobin

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YFP-CL1

   Plasmid

   #11950

   Depositor

   Nico Dantuma

   Article

   Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

   Insert

   YFP-CL1
   

   Expression

   Mammalian

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC57-mitoLbNOX

   Plasmid

   #74448

   Purpose

   This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   mitoLbNOX
   

   Tags

   Flag tag with linker and mitochondrial targeting …

   Mutation

   Human codon optimized

   Available Since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYES2-Ub-M-GFP

   Plasmid

   #11952

   Depositor

   Nico Dantuma

   Article

   Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

   Insert

   Ub-M-GFP
   

   Expression

   Yeast

   Mutation

   Ubiquitin fused to N-terminus of GFP. Methionine …

   Available Since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPAGFP-N1

   Plasmid

   #11909

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   pPAGFP-N1
   

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-N-RAM-d2tTA-TRE-mKate2

   Plasmid

   #140275

   Purpose

   N-RAM, the Npas4-dependent reporter

   Depositor

   Yingxi Lin

   Article

   Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

   Insert

   mKate2
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-iCre

   Plasmid

   #89573

   Purpose

   Expresses iCre under pCAG

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   iCre
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST-ORF-V2

   Plasmid

   #73638

   Purpose

   Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Tags

   Venus fragment 2 (V2)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFR L858R

   Plasmid

   #11012

   Purpose

   Retroviral construct for expressing human EGFR with L858R mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR L858R (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   L858R (tumor-derived)

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1α1.1-GFP

    Plasmid

    #122100

    Purpose

    AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV2

    Insert

    GFP
    

    Use

    AAV

    Tags

    NA

    Expression

    Mammalian

    Promoter

    EF1a（1.1kb short version)

    Available Since

    May 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)

    Viral Prep

    #100798-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Promoter

    Syn

    Tags

    EGFP (Cre-dependent)

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

12. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSyn-DIO-mCherry

    Plasmid

    #50459

    Purpose

    Double floxed mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Promoter

    human Synapsin 1

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-ORF-V1

    Plasmid

    #73637

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS DRR mCherry Donor EF1a BFP

    Plasmid

    #98896

    Purpose

    Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    Neomycin-T2A-mCherry-GFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-LiLac

    Plasmid

    #184570

    Purpose

    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

    Depositor

    Gary Yellen

    Article

    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

    Insert

    LiLac fluorescent sensor of lactate
    

    Use

    AAV

    Tags

    His7

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCas9

    Plasmid

    #42876

    Purpose

    Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    tracr/Cas9
    

    Use

    CRISPR; E.coli

    Expression

    Bacterial

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1-blast

    Plasmid

    #26655

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLumi-LgBiT

    Plasmid

    #188319

    Purpose

    Expression of LgBiT via sigma 70 promoter in E. coli

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    LgBiT
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Bacterial

    Promoter

    sigma 70 promoter

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge