We narrowed to 10,401 results for: 158

1. pHTSHP-ShhN-Cys

   Plasmid

   #121136

   Purpose

   Expresses ShhN with a C-terminal cysteine residues in bacteria

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   shh (Shh Mouse)
   

   Use

   Tags

   HIS, HRV3C site, and MBP

   Expression

   Bacterial

   Mutation

   Amino acid sequence from 26-191, with an addition…

   Promoter

   tac

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Myc-gamma1/gamma2SIL

   Plasmid

   #121432

   Purpose

   GABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG1/GABRG2 SIL (Gabrg1, Gabrg2 Rat)
   

   Use

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. gh151

   Plasmid

   #106821

   Purpose

   expression of gRNA targeting GLT8D1

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   GLT8D1 (GLT8D1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. gh98

   Plasmid

   #106766

   Purpose

   expression of gRNA targeting LFNG

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   LFNG (LFNG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac-GST-Mps1-KD

   Plasmid

   #114066

   Purpose

   protein production

   Depositor

   Geert Kops

   Article

   Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

   Insert

   Mps1-KD (TTK Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Mps1 KD (D664A)

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac-GST-Mps1-WT

   Plasmid

   #114065

   Purpose

   protein production

   Depositor

   Geert Kops

   Article

   Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

   Insert

   Mps1-WT (TTK Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   none

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPN112

   Plasmid

   #91639

   Purpose

   Express sgRNA targeting human MAN2A1

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   MAN2A1 (MAN2A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPN113

   Plasmid

   #91640

   Purpose

   Express sgRNA targeting human MAN2A1

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   MAN2A1 (MAN2A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSN34

   Plasmid

   #100119

   Purpose

   expresses a chimeric OCT4-activator fusion

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   vexGFP-P2A-OCT4 fused to 2 VP16 domains (413-460) separated by linkers (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSN21

    Plasmid

    #100106

    Purpose

    expresses a chimeric OCT4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to a 'weak' VP16 domain (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSN61

    Plasmid

    #100122

    Purpose

    expresses putative OCT4 DBD with a T7 tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-putative OCT4 DBD (138-289) with a T7 tag (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSN35

    Plasmid

    #100120

    Purpose

    expresses a chimeric OCT4-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to an Engrailed domain (168-281) separated by a linker (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSN31

    Plasmid

    #100116

    Purpose

    expresses a chimeric KLF4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-KLF4 fused to a 'medium' VP16 domain (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSN32

    Plasmid

    #100117

    Purpose

    expresses a chimeric KLF4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-KLF4 fused to a 'weak' VP16 domain (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FLAG-Atoh1 del3

    Plasmid

    #96913

    Purpose

    Murine Atoh1 with amino acids 214-305 removed

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 214-305

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-Atoh1 del4

    Plasmid

    #96914

    Purpose

    Murine Atoh1 with amino acids 306-347 removed

    Depositor

    Albert Edge

    Article

    Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 306-347

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSG5-chCAR

    Plasmid

    #87231

    Purpose

    Mammalian expression of chicken CAR

    Depositor

    Fritz Rathjen

    Article

    Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

    Insert

    CXADR (CXADR Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psicheck-2-Mc2r-mod

    Plasmid

    #48173

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of MC2R (MC2R Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    A/G MUTATION IN ATG OF UORF

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

19. psicheck-2-Kdr-mod

    Plasmid

    #48177

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of 2KDR (MC2R Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    A/G MUTATION IN ATG OF UORF

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

20. 5mARF17 pBluescript

    Plasmid

    #12069

    Depositor

    David Bartel

    Article

    Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

    Insert

    5mARF17 (ARF17 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    miR160-resistant version of ARF17 has five silent…

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3.1-Beta3

    Plasmid

    #140988

    Purpose

    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta3 (GNB3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid

    #140980

    Purpose

    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasS-RLuc8
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TRE-Lepr_FL-IRES-eGFP

    Plasmid

    #196635

    Purpose

    Doxycycline inducible expression of full length mouse Lepr with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-GGamma1-GFP2

    Plasmid

    #140989

    Purpose

    Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma1-GFP2 (GNGT1 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-PE7

    Plasmid

    #214812

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mARGAna (cassette 1, transient)

    Plasmid

    #197588

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. plenti-UbcP-Myc-YWAA-pGK-HYG

    Plasmid

    #107377

    Purpose

    Lentiviral expression of myc-YWAA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    CRBN-Y383A/W385A

    Promoter

    UbcP

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. T7_CC_PE7_IVT_Template

    Plasmid

    #223022

    Purpose

    Template for in vitro transcription of PE7. For HSPC-related experiments.

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJT305 Pa01 Lenti pEF-attB LSR GFP

    Plasmid

    #184948

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Ami Bhatt, Patrick Hsu, Michael Bassik, Lacra Bintu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge