We narrowed to 35,220 results for: OMP

1. pcDNA3.1-MS2-HA-HsNot4_1-274_AG

   Plasmid

   #148750

   Purpose

   Mammalian Expression of HsNot4_1-274

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   HsNot4_1-274 (CNOT4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_6HT-TBP6.7(T50A)

   Plasmid

   #112727

   Purpose

   Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(T50A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   T50A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PatoM-OG241

   Plasmid

   #72842

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PatoM

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX_DSB_Spectrum-V3

   Plasmid

   #192476

   Purpose

   Lentiviral DSB Spectrum V3 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, mCherry, incompleteGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX_DSB_Spectrum-V1

   Plasmid

   #192474

   Purpose

   Lentiviral DSB Spectrum V1 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, incompleteGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX_DSB_Spectrum-V2

   Plasmid

   #192475

   Purpose

   Lentiviral DSB Spectrum V2 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, incompleteGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDH_Str-KDEL_ManII-SBP-mCherry

   Plasmid

   #65259

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL0527 (pDonor)

   Plasmid

   #130634

   Purpose

   Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIVM_02

   Plasmid

   #204500

   Purpose

   Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

   Depositor

   Alessio Ciulli

   Article

   Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

   Insert

   amino acids 54-213 VHL (VHL Human)
   

   Use

   Tags

   6xHis, TEV, and Thrombin

   Expression

   Bacterial

   Mutation

   0

   Promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET29b-AK1-His

    Plasmid

    #124135

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Adenylate kinase (AK1 Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LCC-ICCG_pET21b

    Plasmid

    #215408

    Purpose

    Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    LCC-ICCG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F243I/D238C/S283C/Y127G

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Str-KDEL_TNF-SBP-EGFP

    Plasmid

    #65278

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Str-KDEL_ST-SBP-EGFP

    Plasmid

    #65264

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Str-KDEL_TNF-SBP-mCherry

    Plasmid

    #65279

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSD103D

    Plasmid

    #218134

    Purpose

    For competition during selection with hybrid circuit. Expresses MBP fused to evolved degron SD55

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD55
    

    Use

    Tags

    MBP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Str-KDEL_ManII-SBP-mCherry

    Plasmid

    #65253

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TNF-SBP-EGFP_puromycin

    Plasmid

    #65284

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Str-KDEL_ST-SBP-mCherry

    Plasmid

    #65265

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 114_UBI_iCre_crystGFP

    Plasmid

    #171797

    Purpose

    Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Insert

    optimised iCre under the control of the Ubiquitin promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TaRGET-ABE-C3.1 ver4.1

    Plasmid

    #188251

    Purpose

    Hypercompact adenine base editors

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

    Insert

    TaRGET-ABE3.1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Str-KDEL_ManII-SBP-EGFP

    Plasmid

    #65252

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDH_Str-KDEL_ManII-SBP-EGFP

    Plasmid

    #65258

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ST-SBP-mCherry_puromycin

    Plasmid

    #65271

    Purpose

    ST reporter only ( no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pACYC-TtCas6-4xcrRNA4.5

    Plasmid

    #127764

    Purpose

    For expression of T. thermophilus Csm complex in E. coli (crRNA)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Synthetic CRISPR array containing four identical T. thermophilus spacers
    Cas6A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSL0915 (CRISPR with target 3)

    Plasmid

    #130643

    Purpose

    Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    CRISPR(target-3)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDH_TNF-SBP-EGFP

    Plasmid

    #65282

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pT7-EGFP-C1-HsMago_I

    Plasmid

    #146582

    Purpose

    Mammalian Expression of HsMago

    Depositor

    Elisa Izaurralde

    Article

    Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

    Insert

    HsMago (MAGOH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation Y123C compared to the seq…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Str-KDEL_ST-SBP-tagBFP

    Plasmid

    #65266

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-MS2-HA-HsSmg7_T

    Plasmid

    #147556

    Purpose

    Mammalian Expression of HsSmg7

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg7 (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutation G216A, A1521G and one non sil…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Ii-Str_ST-SBP-mCherry

    Plasmid

    #65269

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge