We narrowed to 51,448 results for: son

1. pCHC030 (∆CBBp ∆MBH ∆SH)

   Plasmid

   #226205

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
   

   Use

   Synthetic Biology

   Mutation

   ∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pPB-cT3G-cERH-LHX8

   Plasmid

   #222921

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of LHX8

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   LHX8 (LHX8 Human)
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG8H-GB1-EWS_171-264

   Plasmid

   #180466

   Purpose

   Expresses the construct His8-GB1-EWS_171-264 with a TEV cleavage site between GB1 and EWS_171-264

   Depositor

   David Libich

   Article

   Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   GB1 and His8

   Expression

   Bacterial

   Mutation

   deleted 1-170, 265-656

   Promoter

   T7

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG8H-GFP-EWS_1-120

   Plasmid

   #180464

   Purpose

   Expresses the construct His8-GFP-EWS_1-120 with a TEV cleavage site between GFP and EWS_1-120

   Depositor

   David Libich

   Article

   Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   GFP and His8

   Expression

   Bacterial

   Mutation

   deleted 121f-656

   Promoter

   T7

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJET-62-SFFV-T2A-PuroR-polyA-R11b

   Plasmid

   #179893

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Expression

   Bacterial and Mammalian

   Promoter

   SFFV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJET-64-SFFV-T2A-BlaR-polyA-R11b

   Plasmid

   #179895

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Expression

   Bacterial and Mammalian

   Promoter

   SFFV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Slc1a3-CreERT2 Targeting Vector

   Plasmid

   #129409

   Purpose

   Gene targeting vector to knock-in 2A-CreERT2 into the mouse Slc1a3 locus in place of a STOP codon

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

   Insert

   Slc1a3-2A-CreERT2 Targeting Vector (Slc1a3 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. px335 Slc1a3 nickase 2

   Plasmid

   #129411

   Purpose

   Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

   Insert

   sgRNA targeting Slc1a3 (Slc1a3 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K)

   Plasmid

   #171130

   Purpose

   expresses AMOT(E104K/E105K) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   Angiomotin p130 E104K/E105K (AMOT Human)
   

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   changed glutamic acid residues 104 and 105 to lys…

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-FRT-TO-Nprot_GC3opt

    Plasmid

    #157730

    Purpose

    mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nprot_GC3opt (N SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pcDNA5-FRT-TO-Nsp8_GC3opt

    Plasmid

    #157697

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt

    Plasmid

    #157700

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pcDNA5-FRT-TO-FH-Nsp8_GC3opt

    Plasmid

    #157698

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. KC1G3_HUMAN_D0

    Plasmid

    #79685

    Purpose

    This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1G3 (CSNK1G3 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. LXSN-Cx43-S279A

    Plasmid

    #60705

    Purpose

    retroviral expression of rat Cx43 S279A mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S279A

    Promoter

    LTR

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA-Cx43-S279/282A

    Plasmid

    #60712

    Purpose

    mammalian expression of rat Cx43 S279/282A mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Expression

    Mammalian

    Mutation

    S279/282A

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA-Cx43-S279/282D

    Plasmid

    #60714

    Purpose

    mammalian expression of rat Cx43 S279/282D mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Expression

    Mammalian

    Mutation

    S279/282D

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pEMS1396

    Plasmid

    #29194

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple146 (NTSR1 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS1174

    Plasmid

    #29088

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple240 (UGT8 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEMS1617

    Plasmid

    #29279

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple146 (NTSR1 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades…

    Tags

    Intron-LacZ-NLS

    Available Since

    Oct. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge