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pTol2CG2-QUASM1:GFPNLS-SV40pA

Plasmid

#155123

Purpose

Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pmBaoJin-Tubulin

Plasmid

#229566

Purpose

Expresses mBaoJin-tubulin fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-Cre

Plasmid

#153207

Purpose

Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in retinal ganglion cells

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

mSncg

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ROR2-V5/HIS

Plasmid

#205608

Purpose

Expression of human ROR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROR2 receptor tyrosine kinase (ROR2 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET30a-GABAT

Plasmid

#239572

Purpose

Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Article

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

4-aminobutyrate aminotransferase (gabT E. coli)

Tags

His6

Expression

Bacterial

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta S9A pcDNA3

Plasmid

#14754

Purpose

Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

Depositor

Jim Woodgett

Article

Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

Insert

GSK3 beta S9A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A. Constitutively active mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-7 asyn A53T

Plasmid

#105727

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A53T (SNCA Human)

Expression

Bacterial

Mutation

A53T

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa

Plasmid

#127389

Purpose

Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Tags

mEGFP

Expression

Mammalian

Promoter

pCAG

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α wt-pBabe-Puro

Plasmid

#26055

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA

Plasmid

#155125

Purpose

Tol2 vector containing Cre-inducible QFGal4-switch

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4-switch

Use

Cre/Lox; Zebrafish expression

Promoter

Ubi

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ROS1-V5/HIS

Plasmid

#205609

Purpose

Expression of human ROS1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROS1 receptor tyrosine kinase (ROS1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2050

Plasmid

#49135

Purpose

AAV plasmid with CAGGS promoter driving expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-emGFP

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLM-CMV-R-Cre

Plasmid

#27546

Purpose

Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

Depositor

Michel Sadelain

Article

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

mCherry_P2A_Cre recombinase

Use

Cre/Lox and Lentiviral

Promoter

CMV

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pGWB506

Plasmid

#74848

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)

Plasmid

#106464

Purpose

Expresses Prc1 with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

PRC1

Tags

GFP

Expression

Bacterial and Yeast

Promoter

PRC1

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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mBaoJin-lifeactin

Plasmid

#229608

Purpose

Expresses mBaoJin-lifeactin in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn A30P

Plasmid

#105726

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A30P (SNCA Human)

Expression

Bacterial

Mutation

A30P

Promoter

T7

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB405

Plasmid

#74799

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-NUP98

Plasmid

#229593

Purpose

Expresses EGFP-NUP98 fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

EGFP

Expression

Mammalian

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-GSK3β-#1

Plasmid

#32496

Depositor

Alex Toker

Article

Yoeli-Lerner et al Mol Cancer Res. 2009 Mar;7(3):425-32. Ep

Insert

GSK3B (GSK3B Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only
pBabe puro HA PIK3CA

Plasmid

#12522

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pmBaoJin-Actin

Plasmid

#229609

Purpose

Expresses mBaoJin-actin fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4-NES

Plasmid

#64727

Purpose

Cytosol-targeted, FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

Insert

AKAR4-NES

Tags

Cerulean, NES, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEB3-mBaoJin

Plasmid

#229565

Purpose

Expresses EB3-mBaoJin fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-puro Vps34-GFP

Plasmid

#38268

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

Plasmid

#70231

Purpose

ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB2904(XI-3 MarkerFree)

Plasmid

#73276

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBADHisB-Sumo-mBaoJin

Plasmid

#229580

Purpose

Expresses mBaoJin in bacterial cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Bacterial

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-N-CretrcintG

Plasmid

#69570

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Has Service

AAV1

Insert

kozak consensus - N-CretrcintG

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-C-CreintG

Plasmid

#69571

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Has Service

AAV1

Insert

kozak consensus - C-CreintG

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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