We narrowed to 4,673 results for: GCA

Showing: 2861 - 2880 of 4673 results

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pLKO.1-blast-cfTuba_2

Plasmid

#26718

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Tuba_2 RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLJM1-SLC35B2-FLAG

Plasmid

#154863

Purpose

Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

ctcctggtgcagtacttc => ttattagtccaatatttt

Promoter

CMV

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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SRSF2 A9.3 gRNA

Plasmid

#90902

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRSF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SRSF2 (Guide Designation A9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pX330_ATP1A1_G3_Dual_sgRNA

Plasmid

#173205

Purpose

Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

Dual U6 promoters

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRP_8xgRNA-FLuc

Plasmid

#135937

Purpose

Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

Luciferase

Expression

Mammalian

Promoter

minCMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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CDC6 B5.1 gRNA

Plasmid

#90596

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC6 (Guide Designation B5.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

Plasmid

#124859

Purpose

Mutagenesis of Slc17a7

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a7 (Slc17a7 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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ORC1 G2.1 gRNA

Plasmid

#90821

Purpose

3rd generation lentiviral gRNA plasmid targeting human ORC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ORC1 (Guide Designation G2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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ECT2 D6.1 gRNA

Plasmid

#90673

Purpose

3rd generation lentiviral gRNA plasmid targeting human ECT2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ECT2 (Guide Designation D6.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_CEBPG_2

Plasmid

#86345

Purpose

Encodes gRNA for 3' target of human CEBPG

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against CEBPG (CEBPG Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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MSH6 E8.3 gRNA

Plasmid

#90773

Purpose

3rd generation lentiviral gRNA plasmid targeting human MSH6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MSH6 (Guide Designation E8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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EPHB4-gRNA1/2

Plasmid

#139974

Purpose

gRNA used for knockin EGFP into EPHB4 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EPHB4 gRNA

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Cacnb1 KI

Plasmid

#139660

Purpose

Endogenous tagging of CaVβ1: N-terminal (amino acid position: S8)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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MISP H9.2 gRNA

Plasmid

#90770

Purpose

3rd generation lentiviral gRNA plasmid targeting human MISP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MISP (Guide Designation H9.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-TER+-shLKB1-Ms

Plasmid

#61234

Purpose

Entry clone encoding shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (STK11, Stk11 Human, Mouse)

Use

Entr clone

Promoter

H1/TO

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
MAPRE1 F9.3 gRNA

Plasmid

#90746

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPRE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAPRE1 (Guide Designation F9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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ZW10 H10.3 gRNA

Plasmid

#90943

Purpose

3rd generation lentiviral gRNA plasmid targeting human ZW10

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ZW10 (Guide Designation H10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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MAD2L1 E11.1 gRNA

Plasmid

#90742

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1 (Guide Designation E11.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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CDT1 B10.1 gRNA

Plasmid

#90609

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDT1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDT1 (Guide Designation B10.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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SASS6 G2.3 gRNA

Plasmid

#90885

Purpose

3rd generation lentiviral gRNA plasmid targeting human SASS6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SASS6 (Guide Designation G2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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