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Venus-pBAD

Plasmid

#54859

Purpose

Localization: Protein Expression Vector , Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

6xHis and Venus

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Halo-MTS

Plasmid

#124315

Purpose

Expresses mitochondria specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

Mito specific seq

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pInducer20 Cyclin E1

Plasmid

#109348

Purpose

virus packaged, doxycyline inducible expression of Cyclin E1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cyclin E1 (CCNE1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A-1x3

Plasmid

#58767

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe Nf2 wild-type

Plasmid

#14116

Depositor

Tyler Jacks

Article

Johnson et al Oncogene. 2002 Sep 5. 21(39):5990-7.

Insert

Nf2 (Nf2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pTRE-TIGHT-Cx43-eYFP

Plasmid

#31807

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Cx43 (GJA1 Human)

Tags

eYFP

Expression

Mammalian

Promoter

P-TIGHT

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pJAM1.19_PB-CIT-PGK-Blast

Plasmid

#205564

Purpose

Express human CIT

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

CIT (CIT Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pC1-mitoRexYFP

Plasmid

#60246

Purpose

Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

Depositor

Vsevolod Belousov

Article

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

mitoRexYFP

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFH2.22_mCherry-MS2x8

Plasmid

#205537

Purpose

Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TET-mKLF4

Plasmid

#20906

Purpose

piggyBac vector with tet inducible mKLF4

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

KLF4 (Klf4 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-iC++-EYFP (AAV8)

Viral Prep

#137155-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-iC++-EYFP (#137155). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-iC++-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Promoter

nEF

Tags

EYFP (Cre and Flp-dependent)

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only
pcDNA3-AU1-mTOR-L1460P

Plasmid

#19995

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

mammalian target of rapamycin-L1460P (Mtor Rat)

Tags

AU1

Expression

Mammalian

Mutation

Changed Leucine 1460 to Proline

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#183951

Purpose

Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

APOBEC1 (Apobec1 Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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BICstim-Gag-dCAS9-VPR

Plasmid

#120922

Purpose

encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GAG (FMLV)-dCAS9-VPR

Tags

none

Expression

Mammalian

Promoter

hCMV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

Plasmid

#113884

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

pHR-CMV-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-GFP HDRT Source (pTR 143)

Plasmid

#112012

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-GFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVTREGCaMP6f

Plasmid

#97410

Purpose

Amplified expression of GCaMP6f in combination with TET driver

Depositor

Tetsuo Yamamori

Article

Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

TRE3

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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CAG sHRPb-NLG

Plasmid

#73144

Purpose

Small fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-NLG (Nlgn1 Synthetic, Mouse)

Tags

V5 tag

Expression

Mammalian

Promoter

CAG

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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Ii-Str_TNF-SBP-EGFP

Plasmid

#65280

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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GFP_Rab35 Q67L active

Plasmid

#47425

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 Q67L (RAB35 Human)

Tags

GFP

Expression

Mammalian

Mutation

Q67L active

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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