We narrowed to 19,931 results for: ren

1. pEB1N-LZ-LOV2(wt)

   Plasmid

   #107614

   Purpose

   N-terminal half of π-EB1 with wild-type LOV2, no fluorescent tag

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Use

   Tags

   A. sativa phototropin 1 LOV2 domain and GCN4 leuc…

   Expression

   Mammalian

   Mutation

   EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

   Promoter

   CMV

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-Jun4A-Myc

   Plasmid

   #47444

   Purpose

   mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)

   Depositor

   Axel Behrens

   Article

   Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

   Insert

   Jun 4A mutant (Jun Mouse)
   

   Use

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   four N-terminal residues phosphorylated by JNK, s…

   Promoter

   CMV

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-TWIN-STREP-FLAG-FLCN

   Plasmid

   #164981

   Purpose

   FLCN expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Use

   Tags

   2xSTREP FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-CRY2-P14P5K

   Plasmid

   #79570

   Purpose

   expression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

   Depositor

   Olof Idevall-Hagren

   Article

   Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

   Insert

   Pip5k1c (Pip5k1c Mouse)
   

   Use

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   R445E and R446E; truncation of amino acids 636-66…

   Promoter

   CMV

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pFB-U6-Chrna5-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128340

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrna5 (Chrna5 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1 FLCN (R164A) FLAG GFP

   Plasmid

   #164980

   Purpose

   Lentiviral vector for FLCN expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Use

   Lentiviral

   Tags

   1xFLAG-GFP

   Expression

   Mammalian

   Mutation

   R164A

   Promoter

   UBC

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Gal4-entry+NLS

   Plasmid

   #128013

   Purpose

   Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   Gal4 DBD-SV40NLS-3x-Flag
   

   Use

   Gal4 entry vector with nls to generate tethering …

   Tags

   Gal4-DBD-SV40NLS-3xFlag

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1 FLCN (F118D) FLAG GFP

   Plasmid

   #164979

   Purpose

   Lentiviral vector for FLCN expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Use

   Lentiviral

   Tags

   1xFLAG-GFP

   Expression

   Mammalian

   Mutation

   F118D

   Promoter

   UBC

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGlomyc-Klf4

   Plasmid

   #172870

   Purpose

   Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

   Depositor

   Renee van Amerongen

   Article

   Mourao et al bioRxiv 2021.06.22.449461

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO.1-puro U6 sgRNA Oct4A -158

    Plasmid

    #50922

    Purpose

    U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Oct4A -158 sgRNA (POU5F1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM1 Flag GFP 4E-BP1 Rheb15

    Plasmid

    #112761

    Purpose

    express GFP-4EBP1-Rheb15

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Rheb15

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-nAChr-Alpha6

    Plasmid

    #48812

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pyr pUAST-HA

    Plasmid

    #69768

    Purpose

    Pyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    Klingseisen et al Development. 2009 Jul;136(14):2393-402.

    Insert

    Pyramus (FGF8like-2) (pyr Fly)
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA-Tag

    Expression

    Insect

    Mutation

    241T amino acid insertion compared to reference s…

    Promoter

    hsp70 promoter

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

    Plasmid

    #107730

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

    Plasmid

    #107729

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-CRY2-P14P5K

    Plasmid

    #79569

    Purpose

    expression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

    Depositor

    Olof Idevall-Hagren

    Article

    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

    Insert

    Pip5k1c (Pip5k1c Mouse)
    

    Use

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    R445E and R446E; truncation of amino acids 636-66…

    Promoter

    CMV

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pPRIME-CMV-GFP-shDyn2

    Plasmid

    #132712

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shDyn(2) (Pdyn Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTriEx4-ADCY6-C2 (917-1165 aa)

    Plasmid

    #113904

    Purpose

    His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)
    

    Use

    Tags

    His-S

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Please see Depositor Comments

    Promoter

    T7, CMV

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTriEx4-ADCY6-C1(306-672 aa)

    Plasmid

    #113903

    Purpose

    His-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)
    

    Use

    Tags

    His-S

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7, CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. b2AR->M71-IRES-tauGFP-ACNF TV

    Plasmid

    #105198

    Purpose

    targeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.

    Depositor

    Peter Mombaerts

    Article

    Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

    Insert

    b2AR->M71-IRES-tauGFP-ACNF (Adrb2, Olfr151 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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