We narrowed to 30,126 results for: ren

1. pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

   Plasmid

   #98932

   Purpose

   Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-puro U6 sgRNA Oct4A -12

   Plasmid

   #50921

   Purpose

   U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   Oct4A -12 sgRNA (POU5F1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. LN-entry+NLS

   Plasmid

   #128011

   Purpose

   Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   LN-3xFlag-SV40NLS
   

   Use

   Lamdan entry vector with nls to generate tetherin…

   Tags

   LN-3xFlag-SV40NLS

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. hPlekhm1-dsRedM

   Plasmid

   #73592

   Purpose

   Express human Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   dsRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40

   Plasmid

   #98931

   Purpose

   Cre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPO UBC

   Plasmid

   #68456

   Purpose

   TOPO construct expressing UBC for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Ubiquitin C Promoter (UBC Human)
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

   Plasmid

   #107727

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

   Plasmid

   #127790

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-B-pCR

   Plasmid

   #46369

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

   Insert

   calretinin promoter (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   promoter

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

10. HEL binding scFv D44.1

    Plasmid

    #111718

    Purpose

    Yeast Surface Display of D44.1 as a reference starting point for design

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    D44.1
    

    Use

    Tags

    cmyc tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

    Plasmid

    #107728

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Gal4-entry

    Plasmid

    #128014

    Purpose

    Vector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    Gal4-DBD-3xFlag
    

    Use

    Gal4 entry vector

    Tags

    Gal4-DBD-3xFlag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-HisA-HOXC6-1

    Plasmid

    #110366

    Purpose

    Mammalian expression of HisA-tagged HOXC6-1

    Depositor

    Carlos Moreno

    Article

    Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

    Insert

    HOXC6 (HOXC6 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    long isoform

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5 Flag RagC(GD)/RagB(CTD) chimera

    Plasmid

    #112774

    Purpose

    express Flag-RagC (G domain) fused with with RagB (C terminal domain)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (G domain) fused with RagB (C terminal domain) (RRAGB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5 Flag RagB(GD)/RagC(CTD) chimera

    Plasmid

    #112773

    Purpose

    express Flag-RagB (Gdomain) fused with RagC (C terminal domain)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagB (G domain) fused with RagC (C terminal domain) (RRAGC Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMCSG49-AlleyCat7

    Plasmid

    #42591

    Depositor

    Ivan Korendovych

    Article

    Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

    Insert

    mutated C-terminal portion of calmodulin gene (M76-K148)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Isole…

    Promoter

    T7

    Available since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCVL.SSA TLR (Ani)

    Plasmid

    #45577

    Purpose

    Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with I-Ani I TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Ani I TS from 163-185bp, truncated 25 …

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-HisA-HOXC6-2

    Plasmid

    #110365

    Purpose

    Mammalian expression of HisA-tagged HOXC6-2

    Depositor

    Carlos Moreno

    Article

    Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

    Insert

    HOXC6 (HOXC6 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    short isoform

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. GS2.1/EC

    Plasmid

    #132568

    Purpose

    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    egg cell promoter, Cas9-mApple, pds3 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    A. thaliana egg cell promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128345

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrnb4 (Chrnb4 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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