We narrowed to 18,708 results for: met

1. Yellow Cameleon-Nano65/pcDNA3

   Plasmid

   #51965

   Purpose

   mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano65
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Clomeleon

   Plasmid

   #203723

   Purpose

   A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

   Depositor

   George Augustine

   Article

   Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

   Insert

   cyan fluorescent protein linked yellow florescent protein
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Cyan Fluorescent Protein, Topaz Fluorescent Prote…

   Promoter

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-hEF1A-MG-U6-sgRNA

   Plasmid

   #175505

   Purpose

   All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a, U6

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ii-Str_ManII-SBP-tagBFP

   Plasmid

   #65257

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ii-Streptavidin and truncated Mannosidase II
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and tagBFP

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLM3705

   Plasmid

   #47754

   Purpose

   Expresses mammalian cell codon-optimized dCas9-VP64

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   codon optimized dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9 (catalytically i…

   Promoter

   CMV

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC118-FLIP-FlpE-Puro

   Plasmid

   #84539

   Purpose

   a mock vector for generating FLIP-FlpE targeting vectors

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   FLIP-FlpE - Puro
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV/3' Box_(GLuc)_TOP1

   Plasmid

   #68434

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rogi2-CMV-LAMB3-T2A-GFP-bGH

   Plasmid

   #179865

   Purpose

   Targets Rogi2 genomic region with HDR and expresses LAMB3-T2A-GFP

   Depositor

   George Church

   Article

   Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

   Insert

   LAMB3-T2A-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE102

   Plasmid

   #59397

   Purpose

   MethyloBrick expression vector, mxaF promoter; TcR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PmxaF

   Available Since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-mCherry-LOV2 I427T

    Plasmid

    #81035

    Purpose

    Mammalian expression of LOV I427T, a mutant with super short relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV I427T
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    I427T

    Promoter

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. rASAP

    Plasmid

    #178205

    Purpose

    Ratiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.

    Depositor

    Stefan Heinemann

    Article

    Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

    Insert

    rASAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Yellow Cameleon-Nano15/pcDNA3

    Plasmid

    #51961

    Purpose

    mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pTE100

    Plasmid

    #59395

    Purpose

    MethyloBrick cloning vector, no promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available Since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGal4AD-CIBN

    Plasmid

    #28246

    Purpose

    Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIB1 (1-171aa)
    

    Use

    Tags

    Gal4AD

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRV∆L-5Cre

    Plasmid

    #182964

    Purpose

    The 3rd generation of rabies vector expressing Cre recombinase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    CRE recombinase
    

    Use

    Rabies

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SMVP-Cas9N-U6-gRNA M3

    Plasmid

    #80936

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEos4a-N1

    Plasmid

    #54811

    Purpose

    Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson, Loren Looger

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mEos4a

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAAV-CMV-Cas9C-VPR

    Plasmid

    #80933

    Purpose

    Expresses Cas9C-VPR in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C-VPR
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ910

    Plasmid

    #44481

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, MCS
    

    Use

    Multiple cloning site vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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