We narrowed to 17,410 results for: EMB

1. pEGFP-CHAMP1

   Plasmid

   #159892

   Purpose

   Expresses CHAMP1 (CAMP) in mammalian cells

   Depositor

   Kozo Tanaka

   Article

   Itoh et al EMBO J. 2011 Jan 5;30(1):130-44. doi: 10

   Insert

   CHAMP1 (CHAMP1 Human)
   

   Tags

   pEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5FRT/TO-Strep/HA-UBE2J1

   Plasmid

   #124669

   Purpose

   Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2J1 (UBE2J1 Human)
   

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TagBFP2-INPP4B-CAAX

   Plasmid

   #116857

   Purpose

   plasma membrane-targetted PI(3,4)P2 4-phosphatase

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   INPP4B (INPP4B Human)
   

   Tags

   HRAS(172-189) and TagBFP2

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBrain-GFP-TACC3KDP-shTACC3

   Plasmid

   #59356

   Purpose

   Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

   Depositor

   Stephen Royle

   Article

   Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   RNAi

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acids 30-33 to confer s…

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

   Plasmid

   #188994

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. OMM-long-CFAST10

   Plasmid

   #233598

   Purpose

   Expression of CFAST10 on the OMM membrane after a long linker

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   CFAST10
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1_T7-mScarlet3-Hras

   Plasmid

   #225928

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-Ras membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-HRas
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC-mCh-P2A-ST-EGFP-CAAX

   Plasmid

   #207636

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
   

   Expression

   Mammalian

   Mutation

   Amber stop codon at SpyCatcher’s critical lysine

   Promoter

   CMV

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

   Plasmid

   #190153

   Purpose

   Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV

   Tags

   P2AT2A-EGFP-caax

   Expression

   Mammalian

   Mutation

   Truncation that includes only AA #1-94

   Promoter

   MBP

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSP64-hKCNQ1

    Plasmid

    #53048

    Purpose

    Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)
    

    Use

    Xenopus oocyte expression

    Promoter

    SP6

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2_T7-mScarlet3-KRas

    Plasmid

    #225929

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-KRas
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.CBA.YFP.miR-E_shControl

    Plasmid

    #180386

    Purpose

    Producing AAV that encodes negative control shRNA with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shControl
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-MCS-eDHFR(69K6)-EGFP

    Plasmid

    #172101

    Purpose

    Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    22aa-eDHFR(69K6)-EGFP
    

    Expression

    Mammalian

    Mutation

    eDHFR(69K6): Hexalysine (K6) sequence is inserted…

    Promoter

    CMV

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTH732-2µ-RLuc/staCFLuc

    Plasmid

    #40607

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. NKP90 RFP (787)

    Plasmid

    #62001

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    NKP90 (TMEM126A Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    pgk

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

16. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid

    #227306

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQFlag-USP11 CS puroR

    Plasmid

    #46748

    Purpose

    Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

    Depositor

    Gordon Peters, Goedele Maertens

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKK-TEV-3XFLAG

    Plasmid

    #105784

    Purpose

    Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-3XFLAG

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3_T7-mScarlet3-KRas6R

    Plasmid

    #225930

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-KRas6R
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 10_T7-SP-CD8tm-mScarlet3

    Plasmid

    #225937

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    SP-CD8tm-mScarlet3
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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