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pRST11B-AS3_4

Plasmid

#102789

Purpose

Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Inserts

tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
suppressor tRNA

Expression

Bacterial

Available Since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBS Olfactomedin1

Plasmid

#19225

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Olfactomedin 1 (OLFM1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pCAGGS-PSD95-GCaMP2

Plasmid

#18931

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

PSD95-GCaMP2 (Dlg4 Rat)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-TRP1

Plasmid

#41601

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

13Myc and yeast ADH1 terminator

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP(S65T)-TRP1

Plasmid

#41597

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (S65T) and yeast ADH1 terminator

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3-LRF

Plasmid

#66936

Purpose

Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

Depositor

Alvaro Monteiro

Article

Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV-40

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pTC150

Plasmid

#70014

Purpose

'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Donor + GVR

Expression

Plant

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b-irp3 WT

Plasmid

#87106

Purpose

expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

Insert

irp3 WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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LeGO-iG2-wPRE-pA

Plasmid

#60489

Purpose

eGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)

Depositor

Boris Fehse

Article

Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

Insert

IRES-eGFP; wPRE; BGH-pA

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Plk4(Sak) D154A (Nigg HR26)

Plasmid

#41164

Depositor

Erich Nigg

Article

Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

Insert

Plk4 D154A (PLK4 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

D154A

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGDP1 stat

Plasmid

#112886

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

stat

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCMU-AUTr

Plasmid

#61204

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Positron-ST

Plasmid

#129254

Purpose

Express soma-localized Positron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Gq sensor

Plasmid

#137810

Purpose

A FRET sensor for Gq activation, encoded on a single plasmid

Depositor

Dorus Gadella

Article

Goedhart et al PLoS One. 2011;6(11):e27321. doi: 10.137

Insert

Gq sensor

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Tus pGST-//

Plasmid

#53305

Purpose

Expresses GST-tagged E.coli replication terminator protein Tus.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

E.Coli Tus replication terminator protein

Tags

GST

Expression

Bacterial

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCH1 - pBait-empty

Plasmid

#174663

Purpose

arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.

Depositor

Katherine Berry

Article

Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

Type

Empty backbone

Expression

Bacterial

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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NCD-GFP

Plasmid

#24507

Depositor

Ron Vale

Article

Case et al Cell. 1997 Sep 5. 90(5):959-66.

Insert

NCD (ncd Fly)

Tags

GFP and His

Expression

Bacterial

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
Launchpad (AVA2590)

Plasmid

#85442

Purpose

Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

Depositor

Joan Steitz

Article

Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

Insert

FRT-flanked EF1alfa-GFP PGK-Puromycin cassette

Use

Lentiviral

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only
pMUT2-pLac-mCherry

Plasmid

#192859

Purpose

pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

lldR operon

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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UAS::TVA

Plasmid

#164487

Purpose

Expression of TVA

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Insert

TVA (CD320 Chicken)

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pEM705 Gso (Cys)

Plasmid

#109375

Purpose

GsX chimera with Gαo C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gso chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-mKate2 no-pA

Plasmid

#80828

Purpose

P2A followed by mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-mKate2 no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST53-mTrific

Plasmid

#51031

Purpose

mammalian expression of mouse Trific fused to GFP

Depositor

Timothy Cox

Article

Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

Insert

Trific (Trim46 Mouse)

Tags

Cycle3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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R4227X-F

Plasmid

#21371

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG (following D4226)

Expression

Mammalian

Mutation

Changed R4227 to stop codon

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pBAD/HisB (TIREVOL)-mNG

Plasmid

#189722

Purpose

titratable expression of mNeonGreen in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mNeonGreen

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRsetB-RoTq-Off

Plasmid

#234466

Purpose

Bacterial expression vector for RoTq-Off

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

Insert

RoTq-Off

Tags

7xHis

Expression

Bacterial

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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Bongwoori-Pos6

Plasmid

#101655

Purpose

Bongwoori with the CC1-Pos6 linker length and composition.(* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

Bongwoori-Pos6

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-LIC

Plasmid

#120247

Purpose

For LIC cloning with N-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA_e-1

Plasmid

#202096

Purpose

Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0R_lacZ

Plasmid

#202095

Purpose

Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCA_o-1

Plasmid

#202091

Purpose

Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJOE7771.1

Plasmid

#135077

Purpose

Mannitol, arabitol, and glucitol-inducible mtlR/PmtlE expression vector

Depositor

Josef Altenbuchner

Article

Hoffmann et al PLoS One. 2015 Jul 24;10(7):e0133248. do

Insert

mtlR

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEXP5-NT/pT7 LasR

Plasmid

#193629

Purpose

Constitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.

Depositor

Dora Tang

Article

Gonzales et al ChemSystemsChem 2023

Insert

LasR

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNAP promoter

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5390-CRISPRai-URA3

Plasmid

#182829

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-hPGK_NKX2-1

Plasmid

#221494

Purpose

NKX2-1 short isoform lentiviral overexpression using hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

hPGK

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH4723

Plasmid

#178899

Purpose

Pges-1 DAF-2 unc-54 3' UTR C.elegans gut expression of of DAF-2

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pges-1

Available Since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pTYF-mCMV/SYN-EGFP

Plasmid

#19975

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

mCMV/SYN-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD3

Plasmid

#118043

Purpose

GoldenBraid2.0 Universal Part Domesticator #3

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK316

Plasmid

#235474

Purpose

Inducible gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-T7-VHH72

Plasmid

#202487

Purpose

Expresses the nanobody VHH72 with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJDC124

Plasmid

#157877

Purpose

mCherry is expressed under the mycobacterial phsp60 heat shock promoter for in vivo and in vitro detection.

Depositor

Jeffrey Cirillo

Article

Kong et al PLoS One. 2016 Mar 2;11(3):e0149972. doi

Insert

mCherry

Expression

Bacterial

Promoter

phsp60

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42H-TDH3pr-mNeon

Plasmid

#194539

Purpose

High copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pN-PITCh-HBTH

Plasmid

#127883

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-HBTH

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
M78 pIRESpuro-CBP-2xTEV-StrepTag

Plasmid

#17132

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Tags

2xTEV, CBP, and Streptavidin

Expression

Mammalian

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-1067B

Plasmid

#200252

Purpose

pBac-U6-gRNA(doublesex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

4 gRNAs targeting Doublesex

Expression

Insect

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Myo 1b-myc

Plasmid

#135064

Purpose

Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

Depositor

Martin Bähler

Article

Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

Insert

Myo 1b with 5' UTR (Myo1b Rat)

Tags

Linker and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNC Wnt-3a

Plasmid

#17994

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pRS413-CUP1pr

Plasmid

#221109

Purpose

Copper-inducible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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