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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,310 results for: LEA

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Showing: 2941 - 2960 of 12310 results

  1. NCOR2

    Plasmid
    #40737
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NCOR2 (NCOR2 Human)
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Residues 615 to 685 of the SANT2 domain of Nuclea…
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

    Plasmid
    #169077
    Purpose
    Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-mEGFP-TEV-StrepII
    Depositor
    Sascha Martens
    Publication
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Tags
    HIS10 Tag, TEV cleavage site and mEGFP, GG linker…
    Expression
    Bacterial and Insect
    Promoter
    Polyhedrin promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169077-plasmid-map-sequence-id-332050

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  6. p5T7-LYCipi-ggpps

    Plasmid
    #122017
    Purpose
    Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.
    Depositor
    Gregory Stephanopoulos
    Publication
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Insert
    GGPP synthase
    Expression
    Bacterial
    Mutation
    codon optimized, truncated first 98 amino acids, …
    Promoter
    T7
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122017-plasmid-map-sequence-id-238964

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  9. EGFR-ET1

    Plasmid
    #44185
    Depositor
    Timothy Springer
    Publication
    Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFR-ET1 (EGFR Human)
    Tags
    3C cleavge site, protein C peptide tag, his6, SBP…
    Expression
    Mammalian
    Mutation
    I104V and D254N relative to NP_005219.2
    Promoter
    CMV
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    44185-plasmid-map-sequence-id-458206

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  13. pcDNA3.1(+)-RasAR-NES

    Plasmid
    #205567
    Purpose
    Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.
    Depositor
    Jin Zhang
    Publication
    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RasAR-NES (RAF1 Synthetic, Human)
    Tags
    Nuclear export signal (NES)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205567-plasmid-map-sequence-id-409398

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  14. HA-BACE1-mBFP

    Plasmid
    #196697
    Purpose
    Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-secretase 1 (BACE1 Human)
    Tags
    HA and mBFP
    Expression
    Mammalian
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196697-plasmid-map-sequence-id-386452

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  15. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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Showing: 2941 - 2960 of 12310 results