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TRE-UBC-HLA-G SCT-T2A-GFP

Plasmid

#205462

Purpose

lentiviral plasmid for expression of HLA-G SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HLA-G SCT (HLA-G Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHC01

Plasmid

#106900

Purpose

Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

Depositor

Jonathan Silberg

Article

Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

Inserts

LasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein

Expression

Bacterial

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-FlicR1

Plasmid

#74142

Purpose

Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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Vim-mCh-SspB

Plasmid

#240421

Purpose

Tagged vimentin for optogenetic vimentin pulling experiments.

Depositor

Anna Akhmanova

Article

Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

Insert

Vimentin (VIM Human)

Tags

SspB and mCherry

Expression

Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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p2,0_3M5M_luciferase

Plasmid

#135475

Purpose

Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

Marburg virus minigenome, luciferase reporter

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFBP-2_6.sensor

Plasmid

#162800

Purpose

Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

2_6_RNA-device

Expression

Yeast

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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SDHC-pHLuorin-sems

Plasmid

#107767

Purpose

labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit C of SDH fused to pHluorin (SDHC Human)

Tags

pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMX-eSOX17FNV

Plasmid

#118838

Purpose

Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSOX17FNV (Sox17 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

FNV: L46F, V53N, E57V

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERalpha (ESR1 Human)

Expression

Yeast

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
pNHT7::RPL15::sGluc

Plasmid

#186769

Purpose

An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-DDdCas9VP192-T2A-EGFP-ires-puro

Plasmid

#69534

Purpose

DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

DD-dCas9VP192-T2A-EGFP

Tags

C-term T2A-EGFP and DHFR Destabilised Domain

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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PB-TRE3G-SOX17

Plasmid

#104541

Purpose

PiggyBac vector encoding doxycycline-inducible human SOX17

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

SRY-box 17 (SOX17 Human)

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTACR TH1-p2aGFP

Plasmid

#135815

Purpose

CRISPR based on px330 p2aGFP targeting TH gene near stop codon

Depositor

Tim Ahfeldt, Lee Rubin

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH 1 guide

Use

CRISPR

Tags

p2a Myr-eGFP

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTACR TH2-p2aGFP

Plasmid

#135816

Purpose

CRISPR based on px330 p2aGFP targeting TH gene near stop codon

Depositor

Tim Ahfeldt, Lee Rubin

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH 2 guide

Use

CRISPR

Tags

p2a Myr-eGFP

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pPV-EF1a-mRxxFP1(AfeI)-iP-A

Plasmid

#204625

Purpose

Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Non-specific sgRNA

Plasmid

#109432

Purpose

MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

Non-specific gRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-DEST-MatryoshCaMP6s

Plasmid

#100025

Purpose

Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Tags

V5 Epitope Tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Hsp70-mplum

Plasmid

#45016

Depositor

Anna Planas

Article

de la Rosa et al Eur J Nucl Med Mol Imaging. 2013 Feb;40(

Insert

mPlum

Expression

Mammalian

Promoter

mouse Hspa1b

Available Since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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