We narrowed to 10,398 results for: 158

1. pX022

   Plasmid

   #167148

   Purpose

   MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   C-part of fungal hispidin synthase, nnHispS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX021

   Plasmid

   #167147

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   N-part of fungal hispidin synthase, nnHispS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9

   Plasmid

   #163550

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-HF-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_KARS_tRNA-anti-codon

   Plasmid

   #109865

   Purpose

   Protein expression and purification of KARS_tRNA-anti-codon

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   KARS_tRNA-anti-codon (KARS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PHAGE-P CMVt N-HA hE6-AP I (G20V)

   Plasmid

   #119308

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (G20V) (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   G20V

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PHAGE-P CMVt N-HA hE6-AP I (C21Y)

   Plasmid

   #119304

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (C21Y) (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   C21Y

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)

   Plasmid

   #119313

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 55-376

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PHAGE-P CMVt N-HA hE6-AP I (R39H)

   Plasmid

   #119309

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (R39H) (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   G20V

   Promoter

   CMV

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)

   Plasmid

   #119314

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 55-852

   Promoter

   CMV

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. rat brain inositol 1,4,5-trisphosphate 3-kinase A

    Plasmid

    #122155

    Purpose

    rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)

    Depositor

    Christophe Erneux

    Article

    Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

    Insert

    inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Opto-GPR22

    Plasmid

    #106010

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR22; Opto-GPR22; A12)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR22 (GPR22 Bovine, Human)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_ZDHHC6-1/1

    Plasmid

    #91505

    Purpose

    Protein expression and purification of human SH3 domain construct ZDHHC6-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ZDHHC6-1/1 (ZDHHC6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hASXL(1-115)-luciferase

    Plasmid

    #106427

    Purpose

    Expresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferase

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    addition sex combs like 1 ASXL1 (ASXL1 Human)
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    amino acids 1-115 fused to luciferase

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIneoRL-LFNG 3'UTR-mir200c-mut

    Plasmid

    #84594

    Purpose

    Renilla Luciferase reporter assay for LFNG 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    LFNG 3'UTR (LFNG Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on LFNG 3'…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pMCh-1522

    Plasmid

    #82428

    Purpose

    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED 11W11A
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted and Tr…

    Promoter

    EF1A

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMCh-1526

    Plasmid

    #82429

    Purpose

    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED 11W11A
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted and Tr…

    Promoter

    CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PLXNA4 gRNA (BRDN0001162300)

    Plasmid

    #76878

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLXNA4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLXNA4 (PLXNA4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PLXNA4 gRNA (BRDN0001162265)

    Plasmid

    #76879

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLXNA4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLXNA4 (PLXNA4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PLXNA4 gRNA (BRDN0001145875)

    Plasmid

    #76880

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLXNA4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLXNA4 (PLXNA4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PLXNA4 gRNA (BRDN0001148179)

    Plasmid

    #76881

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLXNA4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLXNA4 (PLXNA4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX-GST-CHD5-WT-FRAG

    Plasmid

    #68873

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    331-672aa

    Promoter

    tac

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

22. KLHL31 pgk (1088)

    Plasmid

    #62031

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    KLHL31 (Klhl31 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

23. 35S:5mARF17 pLBR19

    Plasmid

    #12073

    Depositor

    David Bartel

    Article

    Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

    Insert

    5mARF17 (ARF17 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    miR160-resistant version of ARF17 has five silent…

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pFLAG-YAP1

    Plasmid

    #66853

    Purpose

    Express FLAG-tagged YAP1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFastbac1-Flag-FANCD2

    Plasmid

    #134904

    Purpose

    codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    FANCD2 (FANCD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    polyhedron

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-mTurquoise2-Caspase-1-mNeonGreen

    Plasmid

    #166784

    Purpose

    FRET analysis (pro caspase-1 cleavage) in living cells

    Depositor

    Hao Wu

    Article

    Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

    Insert

    mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 5` CC: Puro – loxP – GFP-C

    Plasmid

    #219563

    Purpose

    5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    hPGK-promoter_PuroR_2A_loxP_GFP-C
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 3` CC: Hygro – GFP-N – loxP – mScarlet

    Plasmid

    #219564

    Purpose

    3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    3` CC: Hygro – GFP-N – loxP – mScarlet
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-GGamma9-GFP2

    Plasmid

    #140991

    Purpose

    Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma9-GFP2 (GNGT2 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmCherry-2xFYVE

    Plasmid

    #140050

    Purpose

    Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge