We narrowed to 10,954 results for: aga

1. LentiCRISPR v2-sgAMPK alpha1 clone3

   Plasmid

   #162121

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106353

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXs-EGFP-ERas SSVA-IP

   Plasmid

   #13827

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

   Insert

   ERas SSVA (Eras Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C224 changed to serine

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

4. WPXL LEFTY2 gRNA

   Plasmid

   #101924

   Purpose

   LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   LEFTY2 enhancer targeting gRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHL 1917

   Plasmid

   #53024

   Purpose

   AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

   Depositor

   Han Lim

   Article

   Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

   Inserts

   mCherry
   fimB (fimB E. coli)
   

   Use

   Synthetic Biology

   Tags

   Asp terminator, RBS(st7), and T1 terminator

   Expression

   Bacterial

   Mutation

   Promoter

   pCon and pLlacO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pClneoMyc' CST-1

   Plasmid

   #66859

   Purpose

   Express Myc-tagged C.elegans CST-1 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

   Insert

   CST-1 (cst-1 Nematode)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S48N, H50R, S257P and V340A mutations in CST-1 co…

   Promoter

   CMV

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pClneoFH-let60 G12V

   Plasmid

   #66860

   Purpose

   Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

   Insert

   Let-60 (let-60 Nematode)
   

   Use

   Tags

   FLAG-His6

   Expression

   Mammalian

   Mutation

   Glycine 12 is mutated to valine

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pORANGE GFP-Cacnb1 KI

   Plasmid

   #139660

   Purpose

   Endogenous tagging of CaVβ1: N-terminal (amino acid position: S8)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA

   Plasmid

   #108844

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV-PM BRD7 sh1

    Plasmid

    #41927

    Depositor

    Stephen Elledge

    Article

    Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pMXs-ERas CVLS-IP

    Plasmid

    #13828

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

    Insert

    ERas CVLS (Eras Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CSVA (224-227) changed to CVLS (CAAX sequence of …

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

12. PCDNA3-N3FLAG-MYB-TAD(251-327)

    Plasmid

    #105611

    Purpose

    transient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAD fragment of MYB (mouse), amino acids 251-327 (Myb Mouse)
    

    Use

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PBK D1.3 gRNA

    Plasmid

    #90824

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PBK

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PBK (Guide Designation D1.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pM-ErbB4CTF-PY3 mutant

    Plasmid

    #17799

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Use

    Tags

    GAL4-BD

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 676-1292, …

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pM-ErbB4-delta-kinase

    Plasmid

    #17800

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Use

    Tags

    GAL4-BD

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 988-1292, …

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

16. INCENP G3.3 gRNA

    Plasmid

    #90712

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human INCENP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    INCENP (Guide Designation G3.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cerulean(1-158) in pcDNAI/Amp

    Plasmid

    #60897

    Purpose

    This vector attaches Cerulean(1-158) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    Cerulean(1-158)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTXB1-xlH2B(1-114)_K114A

    Plasmid

    #118217

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Use

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Mutation

    Lysine 114 to alanine

    Promoter

    T7

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. M26I DJ-1 TEV site pET15b

    Plasmid

    #60685

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26I DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26I and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pmyc-GFP-TNRC6A-(935-984)

    Plasmid

    #42029

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa935-984

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pmyc-GFP-TNRC6A-(1027-1076)

    Plasmid

    #42019

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa1027-1076

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmyc-GFP-TNRC6A-(935-1026)

    Plasmid

    #42018

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa935-1026

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pmyc-GFP-TNRC6A-(925-934)

    Plasmid

    #42017

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa925-934

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pmyc-GFP-TNRC6A-(1077-1709)

    Plasmid

    #42016

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa1077-1709

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YFP Gamma 10

    Plasmid

    #36108

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 10 (Gng10 Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

26. BUB3 A10.1 gRNA

    Plasmid

    #90557

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BUB3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BUB3 (Guide Designation A10.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RGS-6xHis-DRE-1-pcDNA3.1-

    Plasmid

    #52517

    Purpose

    expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Dre-1 (dre-1 Nematode)
    

    Use

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPD95.75-cYAP-1

    Plasmid

    #66857

    Purpose

    Generate transgenic C.elegans with cYAP-1 fused to GFP

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

    Insert

    C.elegans YAP-1 (yap-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Serine104 is mutated to alanine

    Promoter

    C. elegans 5'UTR

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pPD95.75-cYAP-1 S104A

    Plasmid

    #66858

    Purpose

    Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

    Insert

    YAP-1 (yap-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Serine104 is mutated to alanine

    Promoter

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

30. M26L DJ-1 TEV site pET15b

    Plasmid

    #60686

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26L DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26L and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only