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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,110 results for: NSI

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Showing: 5921 - 5960 of 12110 results

  12. pEPQD1CB0776

    Plasmid
    #177066
    Purpose
    MoClo Level 1, position 2, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    strictosidine synthase (CrSTR) from Catharanthus roseus
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177066-plasmid-map-sequence-id-349526

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  13. pEPQD1CB0774

    Plasmid
    #177064
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    tryptophan decarboxylase (CrTDC) from Catharanthus roseus
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177064-plasmid-map-sequence-id-349525

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  14. pEPQD1CB0772

    Plasmid
    #177062
    Purpose
    MoClo Level 1, position 6, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    secologanin synthase (CrSLS1) from Catharanthus roseus
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177062-plasmid-map-sequence-id-349519

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  15. pEPQD1CB0122

    Plasmid
    #177054
    Purpose
    MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177054-plasmid-map-sequence-id-349502

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  16. pEPQD1CB0118

    Plasmid
    #177050
    Purpose
    MoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    iridoid synthase (CrISY) from Catharanthus roseus
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177050-plasmid-map-sequence-id-349498

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  17. pEPQD1CB0116

    Plasmid
    #177048
    Purpose
    MoClo Level 1, position 5, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177048-plasmid-map-sequence-id-349493

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  18. pEPQD1CB0114

    Plasmid
    #177046
    Purpose
    MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    geraniol 8-oxidase (CrG8H) from Catharanthus roseus
    Use
    Synthetic Biology
    Available Since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177046-plasmid-map-sequence-id-349418

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  19. Ash1

    Plasmid
    #169711
    Purpose
    Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)
    Depositor
    Tanja Mittag
    Article
    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323
    Insert
    Ash1 (ASH1 Budding Yeast)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation including encoding amino acids 420-500
    Promoter
    T7
    Available Since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169711-plasmid-map-sequence-id-334782

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  22. psiCHECK2 3'UTR GAC

    Plasmid
    #110401
    Purpose
    Luminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporter
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110401-plasmid-map-sequence-id-227284

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  23. pUC19 KGA HA5'-mKO2-3'

    Plasmid
    #110406
    Purpose
    Homology donor for glutaminase KGA isoform knock-in, mKO2
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Promoter
    none
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110406-plasmid-map-sequence-id-218847

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  24. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available Since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    155388-plasmid-map-sequence-id-324484

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  33. C335

    Plasmid
    #141125
    Purpose
    Expresses chimeric repressor (RbsR-L) under Cin promoter
    Depositor
    Matthew Bennett
    Article
    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:
    Inserts
    LLhR
    AiiA (AQ980_RS12585 Synthetic)
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Chimera: DNA binding domain of LacI and ligand bi…
    Promoter
    engineered cin promoter (responds to C14-HSL) and…
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    141125-plasmid-map-sequence-id-298364

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  34. pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

    Plasmid
    #140223
    Purpose
    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    PGK
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140223-plasmid-map-sequence-id-292003

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Showing: 5921 - 5960 of 12110 results