We narrowed to 13,220 results for: CRISPR-Cas9

1. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

   Plasmid

   #188777

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

   Plasmid

   #188779

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

   Plasmid

   #188778

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

   Plasmid

   #188767

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

   Plasmid

   #62373

   Purpose

   Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
   

   Use

   Tags

   (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…

   Expression

   Bacterial

   Mutation

   Y67S amino acid substitution in (-30)GFP renders …

   Promoter

   T7

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

   Plasmid

   #223148

   Purpose

   Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310

   Promoter

   EF1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

   Plasmid

   #223143

   Purpose

   Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1b (CBX1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 111-173

   Promoter

   EF1a

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBK1308-AAV-EFSNC-dSaCas9-HP1bNH(111-173)

   Plasmid

   #223153

   Purpose

   Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1b (CBX1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 111-173

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBK1313-AAV-EFSNC-dSaCas9-MECP2(204-310)

   Plasmid

   #223158

   Purpose

   Expression of truncated MECP2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

    Plasmid

    #166134

    Purpose

    This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

    Insert

    hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-L3-Cas9-L2

    Plasmid

    #141038

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-L3-Cas9n-L2

    Plasmid

    #141039

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9n
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSEVA231-CRISPR

    Plasmid

    #138712

    Purpose

    Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

    Depositor

    Víctor de Lorenzo

    Article

    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. iF66 PB-LoxStopLox-Zim3-dCas9-mScarlet-hygro

    Plasmid

    #204731

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. iF65 PB-FrtStopFrt-Zim3-dCas9-mScarlet-hygro

    Plasmid

    #204730

    Purpose

    Frt-inducible CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. cTRE-LSL-GFP-IRES-Cas9-sgCR8

    Plasmid

    #135668

    Purpose

    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    sgCR8
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid

    #135669

    Purpose

    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    sgPten
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

    Plasmid

    #138417

    Purpose

    CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Inserts

    BirA
    dCas9
    

    Use

    Lentiviral

    Tags

    FLAG, Avi-tag and V5, His

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

    Plasmid

    #192160

    Purpose

    Dual AAV vector AAV-dCas9

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Insert

    Dead Cas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

    Plasmid

    #208399

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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