Addgene

We narrowed to 21,140 results for: tes

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Rab6bb cds in pGem-T Easy

Plasmid

#80521

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab6bb (rab6bb Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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Rab4a cds in pGem-T Easy

Plasmid

#80513

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab4a (rab4a Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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Rab23 cds in pGem-T Easy

Plasmid

#80542

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab23 (rab23 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRAJ12f

Plasmid

#39248

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system (no terminator for the transRNA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1+pLtetO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ11-12

Plasmid

#39258

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

transRAJ11+cisRAJ12

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1+pLtetO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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HPV16 E7 TCR

Plasmid

#122728

Purpose

Express HPV16 E7 TCR

Depositor

Christian Hinrichs

Article

Jin et al JCI Insight. 2018 Apr 19;3(8). pii: 9948

Insert

HPV16 E7 TCR

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCSN075

Plasmid

#166732

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS-Mxi1

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTalpha1-iCre

Plasmid

#133924

Purpose

Neuron-specific expression of iCre

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

iCre

Use

Tags

Expression

Mammalian

Mutation

Codon optimized

Promoter

neuron-specific tubulin-alpha-1 promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2-P2A-EGFP

Plasmid

#108896

Purpose

Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-P2A-EGFP

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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N-Cadherin-EGFP

Plasmid

#18870

Depositor

Valeri Vasioukhin

Article

Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

Insert

N-Cadherin (Cdh2 Mouse)

Use

Tags

EGFP, Kanamycin, and N-Cadherin

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-H2B-PAmCherry

Plasmid

#133419

Purpose

Fluorescent reporter for calcium imaging and photoactivation

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 WT

Plasmid

#199573

Purpose

Expresses FLAG-tagged WT SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-CaaX(Hras)

Plasmid

#108886

Purpose

Expression of membrane localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry-CaaX(Hras)

Use

Can be used for mammalian expression or in vitro …

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-loxSTOPlox-LynEGFP

Plasmid

#133922

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombination

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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114_UBI_iCre_crystGFP

Plasmid

#171797

Purpose

Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Insert

optimised iCre under the control of the Ubiquitin promoter

Use

Tags

Expression

Mutation

Promoter

Ubiquitin

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-tdTomato-FlagAkt1

Plasmid

#64831

Purpose

Lentiviral expression of murine Flag-Akt1

Depositor

Eva Gonzalez

Article

Kajno et al Biochem J. 2015 Apr 9.

Insert

Akt1 (Akt1 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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H2B-mCherry

Plasmid

#108880

Purpose

Expression of human histone2B fused to mCherry (nuclear marker)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

H2B-mCherry

Use

Can be used for mammalian expression or in vitro …

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH RAP80

Plasmid

#27501

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

RAP80 (UIMC1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2011

Availability

Academic Institutions and Nonprofits only

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pME-secAnxaV-NS

Plasmid

#67718

Purpose

Gateway entry clone containing secreted human AnxaV with no stop codon

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

secAnxaV-NS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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