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FLAG-EAF pBacPAK8

Plasmid

#15323

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor (EAF1 Human)

Use

Baculovirus construction

Tags

6x His and FLAG

Promoter

polyhedrin

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.1-Pct5.1-crRNA(dadR)

Plasmid

#191634

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-NL8F100

Plasmid

#190852

Purpose

For mammalian expression of NL8F100, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

NL8F100 (A Dual-Specificity aptamer targeting OGT and β-catenin)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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NHA-Delta 12

Plasmid

#36439

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A Delta 12 (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 896-1219 only

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-HA-CaMKKbeta rat FL WT

Plasmid

#33321

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

HA

Expression

Mammalian

Mutation

S3V, G96R, and P108L

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pSilencer2.1-U6-3JB1R+6

Plasmid

#190842

Purpose

For mammalian expression of 3JB1R+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

3JB1R_AP3+6bp (A Dual-Specificity aptamer targeting OGT and GFP)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
NHA- Delta 5

Plasmid

#36440

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A Delta 5 (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 1670-1962 only

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

Plasmid

#149594

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Cdc42 T17N sp64T

Plasmid

#16672

Depositor

De-Li Shi

Article

Djiane et al Development. 2000 Jul . 127(14):3091-100

Insert

Cdc42 T17N (CDC42 Human)

Use

Xenopus expression

Mutation

T17N. Dominant negative.

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-ELL2 pBacPAK8

Plasmid

#15327

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

Eleven Nineteen Lysine Rich in Leukemia 2 (ELL2 Human)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA CrkII Y221F

Plasmid

#50732

Purpose

mammalian expression of CrkII Y221F mutant

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII Y221F (CRK Human)

Expression

Mammalian

Mutation

Y221F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
2Bc-T MBP_BoMoC(ed)_I770A_6xH

Plasmid

#185711

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed I770 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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ATF4 15: 5’ATF4.uORF1stopm1.GFP

Plasmid

#21862

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Stop codon for uORF1 converted to UCG (see author…

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pGL3-Control-E2F-3'UTR-Mut

Plasmid

#21171

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

E2F 3'UTR Mutant (E2F1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

2 miR binding sites mutated. Changed from GCACTTT…

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
FLAG-EAF2 pBacPAK8

Plasmid

#15326

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor 2 (EAF2 Human)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pPPC027.306

Plasmid

#171149

Purpose

Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 and J3-BBa_J23117

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-MCPIP1(150-453)

Plasmid

#90109

Purpose

Mammalian expression of Flag-MCPIP1(150-453)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-CαN(E12,14K)-mPAGFP

Plasmid

#168495

Purpose

Mammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3-K83,88,104A

Plasmid

#58405

Purpose

Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysines 83, 88, and 104 to Alanine

Promoter

CMV IE

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83A

Plasmid

#58401

Purpose

Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 83 to Alanine

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only