238,968 results

1. pHR_PGK_antiCD19_synNotch_Gal4VP64

   Plasmid

   #79125

   Purpose

   Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   antiCD19_synNotch_Gal4VP64
   

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138151

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR_BsmBI
   

   Use

   Lentiviral

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-EGFP (AAV8)

   Viral Prep

   #50457-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   EGFP (Cre-dependent)

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pCPRDEnv

   Plasmid

   #1732

   Purpose

   This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

   Depositor

   Garry Nolan

   Insert

   Gag-Pol and Rev
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCPRDEnv

   Plasmid

   #1732

   Purpose

   This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

   Depositor

   Garry Nolan

   Insert

   Gag-Pol and Rev
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHN764

   Plasmid

   #178819

   Purpose

   Expression of EndoH in E. coli

   Depositor

   Kazuhiro Abe

   Insert

   synthetic gene encoding endo-β-N-acetylglucosaminidase H
   

   Tags

   HIs8 and MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-Traptavidin

   Plasmid

   #24753

   Depositor

   Mark Howarth

   Article

   Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

   Insert

   Traptavidin
   

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Streptavidin with 2 amino acid mutations: S52G an…

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-Traptavidin

   Plasmid

   #24753

   Depositor

   Mark Howarth

   Article

   Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

   Insert

   Traptavidin
   

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Streptavidin with 2 amino acid mutations: S52G an…

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHSE401

   Plasmid

   #62201

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHSE401

    Plasmid

    #62201

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEX-DTR-GFP (AAV2)

    Viral Prep

    #124364-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-FLEX-DTR-GFP (#124364). In addition to the viral particles, you will also receive purified pAAV-FLEX-DTR-GFP plasmid DNA. CBA-driven, Cre-dependent expression of DTR-GFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Eiman Azim, Thomas Jessell

    Article

    Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

    Promoter

    Chicken B-actin

    Tags

    GFP (Cre-dependent)

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

12. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207860

    Purpose

    AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmVen-DHX9

    Plasmid

    #211409

    Purpose

    mammalian expression of human DHX9 N-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    DHX9 (DHX9 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 6xHis-SNAP-PI(4)P Probe

    Plasmid

    #211509

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    SidC (P4C)
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUGW-APPSwe-T2A-mCherry

    Plasmid

    #190804

    Purpose

    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Article

    Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Mutation

    Swedish (KM670/671NL)

    Promoter

    Ubi C

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-TBG-ER-TurboID

    Plasmid

    #149415

    Purpose

    Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Has Service

    AAV8

    Insert

    ER-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Promoter

    TBG

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3xFlag-eGFP-Flag-SETX

    Plasmid

    #218838

    Purpose

    Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

    Depositor

    Albert La Spada

    Article

    Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

    Insert

    Senataxin (SETX Human)
    

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMA3790

    Plasmid

    #70110

    Purpose

    mtDNA elimination/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    hUNG1 Y147A
    Neo
    

    Tags

    None, human OTC mitochondrial targeting sequence,…

    Expression

    Mammalian

    Mutation

    Y147A, aa's 1-77 removed

    Promoter

    CMV, EF1a, and SV40 and native

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMA3790

    Plasmid

    #70110

    Purpose

    mtDNA elimination/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    hUNG1 Y147A
    Neo
    

    Tags

    None, human OTC mitochondrial targeting sequence,…

    Expression

    Mammalian

    Mutation

    Y147A, aa's 1-77 removed

    Promoter

    CMV, EF1a, and SV40 and native

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-UbC-Tet1v4-dCas9

    Plasmid

    #232180

    Purpose

    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1-IGF1R-V5/HIS

    Plasmid

    #201979

    Purpose

    expression of human IGF1R receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    IGF1R receptor tyrosine kinase (IGF1R Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPAGFP-N1

    Plasmid

    #11909

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

    Has Service

    Cloning Grade DNA

    Insert

    pPAGFP-N1
    

    Expression

    Mammalian

    Mutation

    These plasmids are essentially the same as the Cl…

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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23. pPAGFP-N1

    Plasmid

    #11909

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

    Has Service

    Cloning Grade DNA

    Insert

    pPAGFP-N1
    

    Expression

    Mammalian

    Mutation

    These plasmids are essentially the same as the Cl…

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCX137

    Plasmid

    #229212

    Purpose

    CRISPR array plasmid for NOTCH2 (Spacers 1-24)

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    CRISPR array plasmid for NOTCH2 (Spacers 1-24)
    

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pRK5-HA-Ubiquitin-K48R

    Plasmid

    #17604

    Purpose

    Mammalian expression of HA tagged ubiquitin K48R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48R (expected to disrupt G76-K48 ubiquitin chain…

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRK5-HA-Ubiquitin-K48R

    Plasmid

    #17604

    Purpose

    Mammalian expression of HA tagged ubiquitin K48R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48R (expected to disrupt G76-K48 ubiquitin chain…

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJRH-1362 scFv entry plasmid

    Plasmid

    #201912

    Purpose

    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3-Lyso-TORCAR

    Plasmid

    #64929

    Purpose

    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-TORCAR (LAMP1 Synthetic, Human)
    

    Tags

    Cerulean, Full-length LAMP1, and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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