164,391 results

1. pcDNA3 p53 S15A

   Plasmid

   #69004

   Purpose

   mammalian expression of human p53 S15A mutant.

   Depositor

   David Meek

   Article

   Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S15A

   Promoter

   CMV

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

   Plasmid

   #129405

   Purpose

   circular Pepper RNA

   Depositor

   Samie Jaffrey

   Article

   Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

   Insert

   circular F30-Pepper(TAR Variant-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmTurquoise2-Mito

   Plasmid

   #36208

   Purpose

   In vivo visualization of the mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   MTS (COX8A Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. Anti-DYKDDDDK [IPI-4E11/M1] (Recombinant Antibody)

   Antibody

   #218106-rAb

   Purpose

   Anti-DYKDDDDK (Flag) tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds in a calcium-dependent manner to the N-terminal DYKDDDDK sequence.

   Depositor

   Institute for Protein Innovation (IPI)

   Article

   Hopp et al Bio/Technology volume 6, pages 1204–1210

   Recommended Applications

   Western Blot

   Reactivity

   Synthetic

   Source Species

   Rabbit

   Isotype

   IgG1

   Trial Size

   Not available to purchase

   Available since

   May 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

5. hIR-GFP

   Plasmid

   #22286

   Depositor

   Joseph Bass

   Article

   Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

   Insert

   INSR insulin receptor (INSR Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. TL028_AAV_hSyn:HaloTag-GluA1

   Plasmid

   #192520

   Purpose

   Expression of AMPA GluA1 with N terminal HaloTag fusion

   Depositor

   Adam Cohen

   Article

   Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

   Insert

   HaloTag-GluA1
   

   Use

   AAV and Cre/Lox

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-15F11-HA-Halo

   Plasmid

   #129592

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRK-FLAG-UbxD8

   Plasmid

   #53777

   Purpose

   express FLAG-tagged human UbxD8 gene

   Depositor

   Yihong Ye

   Article

   Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

   Insert

   UbxD8 (FAF2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pAAV.CAG.(mito).cpSFGFP.HaloTag

   Plasmid

   #214925

   Purpose

   Expresses mitochondrially targeted non-responsive control

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (mito).cpSFGFP.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Anti-Strep [IPI-C23.21] (Recombinant Antibody)

    Antibody

    #218105-rAb

    Purpose

    Anti-Strep tag chimeric recombinant antibody, with fused mouse variable and rabbit constant domains; binds to WSHPQFEK sequence.

    Depositor

    Institute for Protein Innovation (IPI)

    Article

    Institute for Protein et al Unpublished

    Recommended Applications

    Western Blot

    Reactivity

    Source Species

    Rabbit

    Isotype

    IgG1

    Trial Size

    Not available to purchase

    Available since

    May 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

11. pEAK-Flag/INPP4B

    Plasmid

    #24324

    Depositor

    Lewis Cantley

    Article

    Gewinner et al Cancer Cell. 2009 Aug 4. 16(2):115-25.

    Insert

    Inositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    May 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Fab_8D3_2_H-H6

    Plasmid

    #176075

    Purpose

    Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    His6 tagged heavy chain of Fab_8D3_2
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Fab_8D3_2_L

    Plasmid

    #176076

    Purpose

    Express light chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    Light chain of Fab_8D3_2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-GFP

    Plasmid

    #86537

    Purpose

    Retrovirus expressing GFP

    Depositor

    Xue Han

    Article

    Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin I (human)

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-luciferase

    Plasmid

    #55764

    Purpose

    luciferase reporter for in vivo bioluminescence imaging

    Depositor

    Snorri Thorgeirsson

    Article

    Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

    Insert

    CMV enhancer/chicken beta actin promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer/chicken beta actin promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CNR1-DuET

    Plasmid

    #213213

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CNR1 (CNR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-PIK3C2B

    Plasmid

    #161989

    Purpose

    Mammalian expression vector containing PIK3C2B tagged with GFP

    Depositor

    Volker Haucke, David Bryant

    Article

    Marat et al Science. 2017 Jun 2;356(6341):968-972. d

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    siRNA resistance to siRNA#2 with 4 silent mutatio…

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSQT1313

    Plasmid

    #53370

    Purpose

    multiplex gRNA expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    None
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Synthetic, Human)
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-mDlx-GCaMP6f-Fishell-2 (AAV1)

    Viral Prep

    #83899-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-mDlx-GCaMP6f-Fishell-2 (#83899). In addition to the viral particles, you will also receive purified pAAV-mDlx-GCaMP6f-Fishell-2 plasmid DNA. mDlx-driven expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    mDlx

    Tags

    None

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

22. pBABE-hygro-hTERT

    Plasmid

    #1773

    Purpose

    Retroviral expression of hTERT. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ALFA-bArr1

    Plasmid

    #201495

    Purpose

    Expression of the beta-arrestin1 containing a N-terminal ALFA tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    ALFA-bArr1 (ARRB1 Human)
    

    Use

    Tags

    ALFA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBAD-HisD-miniGFP2

    Plasmid

    #188897

    Purpose

    Express 6xHis-Tag miniGFP2 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAVS1 SA-2A-puro-pA donor

    Plasmid

    #22075

    Purpose

    AAV zinc finger donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    SA-Puro
    

    Use

    Zfn donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

    Plasmid

    #60904

    Purpose

    The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-parkin WT

    Plasmid

    #45875

    Depositor

    Edward Fon

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBA560

    Plasmid

    #186236

    Purpose

    pTet-eLbuCas13a crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

    Plasmid

    #171019

    Purpose

    Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    LargeBit-SNAPtag- Beta-actin
    

    Use

    Lentiviral and Luciferase

    Tags

    Beta actin and LargeBiT

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge