Skip to main content
Addgene
Showing: 4501 - 4530 of 160434 results

  4. pC5Kan-P2A

    Plasmid
    #51814
    Purpose
    P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message
    Depositor
    Barry Ganetzky
    Article
    Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:
    Insert
    P2A peptide (PTV1gp1 porcine teschovirus-1, Synthetic)
    Use
    Shuttle vector for insect expression vectors
    Tags
    Expression
    Mutation
    codons altered for Drosophila and to eliminate re…
    Promoter
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    51814-plasmid-map-sequence-id-79554

    Enlarge

  5. pGL4_CRE-CMVmin-luc2

    Plasmid
    #194384
    Purpose
    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter
    Depositor
    Michael Wehr
    Article
    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48
    Insert
    6xCRE-CMVmin-luc2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV minimal
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194384-plasmid-map-sequence-id-381615

    Enlarge

  8. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

    Enlarge

  9. pEGFP rbFOX1

    Plasmid
    #63085
    Purpose
    Mammalian expression of EGFP tagged FOX1
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    RBFOX1 (RBFOX1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63085-plasmid-map-sequence-id-104484

    Enlarge

  11. pILGFP3AA5

    Plasmid
    #185875
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185875-plasmid-map-sequence-id-369502

    Enlarge

  12. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV9)

    Viral Prep
    #100845-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Tags
    None
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV PHP.eB)

    Viral Prep
    #104491-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Tags
    None
    Available since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. TFORF0276

    Plasmid
    #142386
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF3 (TCF3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142386-plasmid-map-sequence-id-385936

    Enlarge

  16. pLenti-CMV-CAG-GFP-EnvA(N2c)

    Plasmid
    #194353
    Purpose
    Lentivirus expressing eGFP and (EnvA-N2c Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies of CVS-N2c strain
    Depositor
    Marco Tripodi
    Article
    Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi
    Inserts
    Enhanced Green Fluorescent Protein (eGFP)
    EnvA-CVS-N2c Rabies Glycoprotein
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194353-plasmid-map-sequence-id-391646

    Enlarge

  21. pHR-SIN-BX SpyCatcher003-(short)mCD80

    Plasmid
    #210567
    Purpose
    Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    210567-plasmid-map-sequence-id-417655

    Enlarge

  22. p3E_he1a:eYFP

    Plasmid
    #113879
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    eYFP
    Expression
    Mutation
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113879-plasmid-map-sequence-id-222563

    Enlarge

  29. pICH41331::pU6cm-pegR::EURb7

    Plasmid
    #227695
    Purpose
    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).
    Depositor
    Jae-Yean Kim
    Article
    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41
    Insert
    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    U6 composite
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227695-plasmid-map-sequence-id-452432

    Enlarge

  30. TFORF3159

    Plasmid
    #144635
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SMAD2 (SMAD2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144635-plasmid-map-sequence-id-348301

    Enlarge

Showing: 4501 - 4530 of 160434 results