We narrowed to 16,615 results for: puromycin

1. pLKO.1P shNfs1

   Plasmid

   #102967

   Purpose

   Suppression of mNfs1

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shNfs1
   

   Use

   Lentiviral and RNAi

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-PM-BAF180-SH3

   Plasmid

   #41933

   Depositor

   Stephen Elledge

   Article

   Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   BAF180
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

3. MSCV-PM-BAF180-SH1

   Plasmid

   #41931

   Depositor

   Stephen Elledge

   Article

   Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   BAF180
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

4. MSCV-PM mir shHoxB9 a

   Plasmid

   #27021

   Depositor

   Joan Massague

   Article

   Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

   Insert

   mir HoxB9 2A
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

5. CJ38 (miR-1006 seed mutant perfect site)

   Plasmid

   #17771

   Depositor

   David Bartel

   Article

   Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

   Insert

   miR-1006 seed mutant perfect site
   

   Use

   Luciferase; Drosophila metallothionein gene promo…

   Tags

   renilla luciferase

   Expression

   Insect

   Mutation

   seed mutant

   Available Since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

6. CJ39 (miR-1003 seed mutant perfect site)

   Plasmid

   #17772

   Depositor

   David Bartel

   Article

   Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

   Insert

   miR-1003 seed mutant perfect site
   

   Use

   Luciferase; Drosophila metallothionein gene promo…

   Tags

   renilla luciferase

   Expression

   Insect

   Mutation

   seed mutant

   Available Since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

7. CJ45 (miR-1003 5' splice site mut)

   Plasmid

   #17775

   Depositor

   David Bartel

   Article

   Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

   Insert

   dme-miR-1003 minigene 5' splice mut
   

   Use

   Drosophila metallothionein gene promoter drives e…

   Expression

   Insect

   Mutation

   5' splice site mut + compsensatory stem

   Available Since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

8. PLKO.1-UPF1-3'UTR

   Plasmid

   #136036

   Purpose

   UPF1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (TTATTACCCAGAATAAGATGC)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW-Cas9-2A-EGFP

   Plasmid

   #167928

   Purpose

   Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   T2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   tTRE, hPGK

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-IRES-Puro

    Plasmid

    #85132

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK243 (Tet-OsTIR1-PURO)

    Plasmid

    #72835

    Purpose

    Expresses OsTIR1 under the control of the Tet promoter

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    AAVS1-Tet-OsTIR1-PURO-AAVS1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. plentiCRISPR-sgGOT1

    Plasmid

    #72874

    Purpose

    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgGOT1
    

    Use

    Lentiviral

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

13. mCherry- SEC61B

    Plasmid

    #121160

    Purpose

    Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    SEC61B (SEC61B )
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXPR_050

    Plasmid

    #96925

    Purpose

    lentiviral expression of gRNA scaffold

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe-puro largeTcDNA

    Plasmid

    #14088

    Depositor

    William Hahn

    Article

    Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

    Insert

    Large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEM705 Gsi (Ser)

    Plasmid

    #109352

    Purpose

    GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsi chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cys at position 391 to Serine (Pertussis …

    Promoter

    CAG

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTi4.0_SpdCas9_pmcDA1

    Plasmid

    #204613

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9-sgAAVS1-1

    Plasmid

    #129726

    Purpose

    Expressing SpCas9 and sgAAVS1-1

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    SpCas9 and sgAAVS1-1
    

    Use

    CRISPR

    Tags

    P2A-Puro

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. AICSDP-63: ACTN2-mEGFP

    Plasmid

    #124607

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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