We narrowed to 15,946 results for: LEA
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Plasmid#196528PurposeEntry vector for Gateway with Eps8L2_181-299DepositorInsertEps8L2_181-299 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD
Plasmid#196881PurposeNeuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.DepositorInsertmOrange2-Cdk5rap2-CTD
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-Eps8L2_1-496
Plasmid#196516PurposeExpression of GST-Eps8L2_1-496DepositorInsertEps8L2_1-496 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationSilent mutations for siRNA resistancePromoterlac promoterAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook1
Plasmid#198523PurposeExpression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook1 (HOOK1 Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent substitution in codon 620 to eliminate int…PromoterADH1Available SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
B52_puro_gRNA_3418711_KCNB2
Plasmid#197558PurposegRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)DepositorInsertupstream KCNB2 promoter gRNA (Kcnb2 Rat)
UseGrna with puromycin selectionTagspuromycin resistance cassette under CMV promotionExpressionMammalianMutationPromoterU6Available SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-delLN-DmPABPC1-V5His6_H
Plasmid#146477PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S, and seven silent mu…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_1-183
Plasmid#196535PurposeExpression of GST-Eps8L2_1-183DepositorInsertEps8L2_1-183 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_181-299
Plasmid#196536PurposeExpression of GST-Eps8L2_181-299DepositorInsertEps8L2_181-299 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_544-715
Plasmid#196540PurposeExpression of GST-Eps8L2_544-715DepositorInsertEps8L2_544-715 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nmyc-Eps8L2-FL-siR
Plasmid#196514PurposeExpression of Myc-Eps8L2-FLDepositorInsertEps8L2-FL-siR (EPS8L2 Human)
UseTagsMycExpressionMammalianMutationSilent mutations for siRNA resistancePromoterT7 promoterAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_529-715
Plasmid#196533PurposeEntry vector for Gateway with Eps8L2_529-715DepositorInsertEps8L2_529-715 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_297-370
Plasmid#196537PurposeExpression of GST-Eps8L2_297-370DepositorInsertEps8L2_297-370 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_367-496
Plasmid#196538PurposeExpression of GST-Eps8L2_367-496DepositorInsertEps8L2_367-496 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_494-546
Plasmid#196539PurposeExpression of GST-Eps8L2_494-546DepositorInsertEps8L2_494-546 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCavin1a in pT3TS-Dest
Plasmid#194294PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAIDepositorInsertcavin1a (cavin1a Zebrafish)
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-HsNRF2 (NFE2L2)
Plasmid#194302PurposeMultisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRSDepositorAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-D1
Plasmid#184246PurposeRecombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 Josephin domain and UIM1 (ATXN3 Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationPremature stop codon N263XPromoterT7 promoterAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-HsSyx3
Plasmid#179289PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + GFP + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO5-SgHottip-GFP-CRISPRi
Plasmid#134989PurposedCas9-mediated inactivation of HOTTIP in mammalian cellsDepositorInsertHOTTIP (HOTTIP Human)
UseLentiviralTagsGFPExpressionBacterial and MammalianMutationPromoterU6Available SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-NmClover3-hsLem2
Plasmid#179524PurposeExpress OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoterDepositorAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE
Plasmid#174133PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryExpressionMutationOnly contains the sequences coding for amino acid…PromoterhSynAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE
Plasmid#174135PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryExpressionMutationOnly contains the sequences coding for amino acid…PromoterhSynAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Nr4a1sgRNA2
Plasmid#160946PurposeGuide RNA 2 to generate Nr4a1 knockout by CRISPRDepositorAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)
Plasmid#155010PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationE1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)
Plasmid#155134PurposeExpresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationC104A/D243G/L244G/F245G/M247C/S248G/M249G/A250G, …PromoterT7Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shCrh2
Plasmid#132710PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh geneDepositorAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB61 - pL0_V2 (CDS1)
Plasmid#123183PurposeGolden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005DepositorInsertTomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
UsePart for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sitesPromoterAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_R89A-PolyA
Plasmid#112291PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Arginine 89 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_L91A-PolyA
Plasmid#112292PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Leucine 91 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_F95A-PolyA
Plasmid#112293PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Phenylalanine 95 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met1 diUb (1-152, R42W)
Plasmid#110767PurposeBacterial expression of Met1 diUbDepositorAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mPC1_4119-eGFP-3HA
Plasmid#108393PurposeExpress cytoplasmic tail mouse PC1 from amino acid 4119 to 4293DepositorAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mPC1_4077-3HA
Plasmid#108392PurposeExpress cytoplasmic tail mouse PC1 from amino acid 4077 to 4293DepositorAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mPC1_4077-eGFP
Plasmid#108391PurposeExpress cytoplasmic tail mouse PC1 from amino acid 4077 to 4293DepositorAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)
Plasmid#70667PurposeHuman mutant of E2F1 lacking amino acids 193-359DepositorInsertE2F1 lacking residues 193 to 359 (E2F1 Human)
UseTagsFLAGExpressionMammalianMutationlacks amino acids 193-359PromotercmvAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#16
Plasmid#75253Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#2
Plasmid#75254Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#1
Plasmid#75248Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)
Plasmid#70669PurposeHuman mutant of E2F1 lacking amino acids 284 to 359DepositorInsertE2F1 lacking residues 284 to 359 (E2F1 Human)
UseTagsFlagExpressionMammalianMutationlacks amin acids 284 to 359PromotercmvAvailable SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pde3b_L (OZ591)
Plasmid#35231DepositorInsertZinc finger array targeting pde3b (pde3b Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pde3b_R (OZ592)
Plasmid#35232DepositorInsertZinc finger array targeting pde3b (pde3b Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
LOC566040_ R (OZ588)
Plasmid#35228DepositorInsertZinc finger array targeting LOC566040 (LOC566040 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
fam82a2_L (OZ573)
Plasmid#35213DepositorInsertZinc finger array targeting fam82a2 (rmdn3 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
LOC566040_ L (OZ587)
Plasmid#35227DepositorInsertZinc finger array targeting LOC566040 (LOC566040 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
morc3b_R (OZ576)
Plasmid#35216DepositorInsertZinc finger array targeting morc3b (morc3b Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
fam82a2_R (OZ574)
Plasmid#35214DepositorInsertZinc finger array targeting fam82a2 (rmdn3 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
morc3b_L (OZ575)
Plasmid#35215DepositorInsertZinc finger array targeting morc3b (morc3b Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1710
Plasmid#29296PurposePleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle238 (TRH Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable SinceJune 10, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
xylt1_R (OZ510)
Plasmid#27185DepositorInsertZinc finger array targeting xylt1 (xylt1 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceFeb. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pitrm1_R (OZ516)
Plasmid#27191DepositorInsertZinc finger array targeting pitrm1 (pitrm1 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only