We narrowed to 32,172 results for: uros

1. MSCV-EZH2-PGK-Puro-IRES-GFP

   Plasmid

   #75125

   Purpose

   Retroviral expression plasmid encoding wild type human EZH2

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   KMT6 (EZH2 Human)
   

   Use

   Retroviral

   Tags

   None

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pPB-CAG-dCas9-VPR-wpA-pPuroTK

   Plasmid

   #214127

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-TRE-MyoD-P2A-miRFP703_PGK-Puro

   Plasmid

   #216666

   Purpose

   Expresses tTa-inducible MyoD and miRFP703, with puromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   MyoD (Myod1 Mouse)
   miRFP703
   PuroR
   

   Use

   Lentiviral

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-FLIP-puro-dsRed-GFP-miRNA

   Plasmid

   #32704

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Insert

   emGFP-miRNA
   

   Use

   Cre/Lox, RNAi, and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

   Plasmid

   #193465

   Purpose

   Dn29 Donor with promoter for mcherry and puro expression

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Dn29_attP
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

   Plasmid

   #120561

   Purpose

   Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   KRAS4B (KRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-HIF2α P405A Δ450-572-pBabe-Puro

   Plasmid

   #25958

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Available Since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-puro GFP-p62 K7A, D69A

   Plasmid

   #38281

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Sequestosome-1 (Sqstm1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed K7A, D69A

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

9. PLKO-mcherry-luc-puro-TRPM1 cDNA

   Plasmid

   #29782

   Depositor

   Carl Novina

   Article

   Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

   Insert

   TRPM1 (TRPM1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

    Plasmid

    #226009

    Purpose

    DjCas13d guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(46))-PGKpuroBFP-W

    Plasmid

    #200504

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA4(43), SMARCA2(46) (SMARCA2, SMARCA4 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti PGK Puro GFP-LC3B G120

    Plasmid

    #123241

    Purpose

    Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    PGK

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS

    Plasmid

    #127656

    Purpose

    Over-express NLS-FLAG-cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. N114 nLV EF-1a-Gal4-VP16-PGK-Puro

    Plasmid

    #44014

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    Gal4/DBD (1-147)
    

    Use

    Lentiviral

    Tags

    VP16/Activation/Domain

    Promoter

    EF-1a

    Available Since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tol2 8xSTAR-mScarletI-NLS. PGK-puro

    Plasmid

    #136263

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI-NLS

    Expression

    Mammalian

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. TetO-FUW-BICRAL-FLAG-pgk-puro

    Plasmid

    #107732

    Purpose

    Tet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORF

    Depositor

    Emily Dykhuizen

    Article

    Alpsoy et al J Biol Chem. 2018 Jan 26. pii: RA117.001

    Insert

    BICRAL (BICRAL Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKL038 Lenti U6 dCas12a gRNA Puro mCherry

    Plasmid

    #195546

    Purpose

    dCas12a gRNA expression backbone

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti U6- empty cassette_Direct repeat_puro_mcherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2-hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #225999

    Purpose

    To establish stable dCas9-KRAB knock-in line

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    humanized dCas9-KRAB T2A Puro
    

    Use

    Sleeping beauty transposon

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PLKO-mcherry-luc-puro-Renilla cDNA

    Plasmid

    #29783

    Depositor

    Carl Novina

    Article

    Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

    Insert

    Renilla
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. WT1-2A-eGFP PGK PuroR Donor Plasmid

    Plasmid

    #82333

    Purpose

    WT1 targeting donor

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Inserts

    WT1 5' Arm (WT1 Human)
    WT1 3' Arm (WT1 )
    

    Use

    CRISPR and TALEN

    Tags

    2A-eGFP

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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