We narrowed to 26,294 results for: ERG

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HA-FOXO3a TM-ER-delta DB

Plasmid

#8354

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

T32A, S253A, S315A, ER (G525R), delta DB (deletio…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pBabe human FOXC2

Plasmid

#15535

Depositor

Bob Weinberg

Article

Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

Insert

FOXC2 (FOXC2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
E42_gRunx1_gRNA3_dTet_NGFR

Plasmid

#189803

Purpose

Retroviral delivery of guide RNA against mouse Runx1

Depositor

Ellen Rothenberg

Article

Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

gRunx1_gRNA3 (Runx1 Mouse)

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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L2092-GPA-FullBRET-Retrovirus-TD142

Plasmid

#222877

Purpose

Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-NanoLuc-CyOFP1

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

Plasmid

#172501

Purpose

Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 full locus with Golden gate acceptor site for spacer

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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L2125-FRB-GPA-NLuc-CyOFP1

Plasmid

#222885

Purpose

Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc-10GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-Turbo-NES-YFP-STOP-L2

Plasmid

#127358

Purpose

gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-NLS-Cre-2A-mCherry-FKF

Plasmid

#119868

Purpose

Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Tags

mCherry

Expression

Bacterial

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(H75E)

Plasmid

#137829

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(H75E)

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

Plasmid

#186993

Purpose

AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122, non-targeting crRNA guide

Use

AAV

Mutation

s1006-gggs-d1221 deletion

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4/hL3MBTL1

Plasmid

#28231

Depositor

Danny Reinberg

Article

Trojer et al Cell. 2007 Jun 1. 129(5):915-28.

Insert

l(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Irrelevant S49T mutation

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only
pSL0762 (pQCascade_crRNA-nt)

Plasmid

#130636

Purpose

Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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scAAV-Mlc2v-MPRA

Plasmid

#182649

Purpose

Self complementary AAV vector for high-throughput in vivo measurement of enhancer activity by massively parallel reporter assay

Depositor

William Pu

Article

Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

rat Mlc2v

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pC011 LwCas13a locus with targeting spacer

Plasmid

#91903

Purpose

LwCas13a locus into pACYC184 with targeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a with targeting spacer

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pJR019

Plasmid

#198834

Purpose

Inactivation of G protein subunits through Pertussis toxin (PTX)

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::PTX-SL2-GFP::let-858 3'UTR

Expression

Worm

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro H-Ras G12V T35S

Plasmid

#12274

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V T35S (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The T35S mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3- myc NES-mCE(K294A) NLS

Plasmid

#82467

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NLS deleted from mouse Capping Enzyme (Rngtt Mouse)

Expression

Mammalian

Mutation

deletion of KRKY from 573-657

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2_mScarletI_mVenusNB-MrkHR113A

Plasmid

#182292

Purpose

expresses c-di-GMP mutant biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkHR113A

Tags

mVenusNB internal fusion to MrkH with a R113A poi…

Expression

Bacterial

Mutation

changed arginine 113 to alanine, MrkH numbering …

Promoter

proC

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCVL.Active/Repressed TLR (Sce)

Plasmid

#45575

Purpose

Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

eGFP with I-Sce I TS
+3 mCherry

Use

Lentiviral

Tags

T2A dislinker and iRFP

Expression

Bacterial and Mammalian

Mutation

embedded I-Sce I TS from 163-185

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCAD16_CAD_12-490_pvp008

Plasmid

#124842

Purpose

Expresses Aspergillus terreus CAD in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-11, synthetic codon optimiz…

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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