We narrowed to 5,907 results for: Abo;

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pCMV-PuroR-T2A-eGFP

Plasmid

#234734

Purpose

Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PuroR-T2A-eGFP

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-AAVS1-5-1kbdonor

Plasmid

#234735

Purpose

PseCAST crRNA targeting AAVS1-5, with 1 kb donor transposon

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

AAVS1-5 crRNA

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-PLA2G15-6xHis WT

Plasmid

#213603

Purpose

Expresses PLA2G15-6xHis in mammalian cells

Depositor

Monther Abu-Remaileh

Article

Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

Insert

Phospholipase A2 Group XV (PLA2G15 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-sfGFP

Plasmid

#116917

Purpose

Strong constitutive expression of super-folder GFP for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

J23101

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCph8

Plasmid

#22869

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2005 Nov 24. 438(7067):441-2.

Insert

pCph8

Use

Synthetic Biology

Expression

Bacterial

Mutation

First 517 amino acids of Cph1 and the last 229 am…

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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myc-BioID2-MCS

Plasmid

#74223

Purpose

To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag

Depositor

Kyle Roux

Article

Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

Myc and humanized A. aeolicus BioID2 with R40G

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9cur

Plasmid

#71538

Purpose

Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

Depositor

Tao Chen

Article

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Inserts

Cas9
Plasmid curing system

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX

Plasmid

#111516

Purpose

Expresses E. coli ClpX with a C-terminal His tag

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-5ptaseOCRL

Plasmid

#66836

Purpose

Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

5ptaseOCRL (OCRL Human)

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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Lyn11-CIBN-GFP

Plasmid

#79572

Purpose

expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

Lyn11

Tags

CIBN (CRY2-binding domain) and EGFP

Expression

Mammalian

Mutation

G8R (please see depositor comments below)

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
mycBioID2-13X Linker-MCS

Plasmid

#92308

Purpose

To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.

Depositor

Kyle Roux

Article

Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

Humanized A. aeolicus BioID2 with R40G and Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCHC005 (∆RE)

Plasmid

#226199

Purpose

Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for type I restriction-modification system restriction subunit H16_A0006

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDY1440 STITCHR GFP NOLC1 payload

Plasmid

#234829

Purpose

GFP cargo with 50bp NOLC homology arms

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

copGFP

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(ΔGTPase)- GFP

Plasmid

#246843

Purpose

Express Arl13B (resistance to sgRNA) with deletion of GTPase, tagged with GFP

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted GTPase domain

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-YopE

Plasmid

#221328

Purpose

Dox-inducible expression of YopE in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

YopE

Use

Retroviral

Tags

FLAG

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBS MCK promoter

Plasmid

#12528

Depositor

Ronald Kahn

Article

Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

Insert

MCK promoter (Ckm Mouse)

Expression

Mammalian

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pENTR-PPP2R2B

Plasmid

#16181

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

PP2A regulatory subunit B, beta isoform (PPP2R2B Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pCHC042 (∆phaCAB)

Plasmid

#226208

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for phaCAB

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

Plasmid

#154003

Purpose

Vector expressed CasRx and sgRNA_LACZ for AAV package

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

CasRx

Use

AAV and CRISPR

Promoter

EFS

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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