We narrowed to 11,195 results for: ENA

1. pCAT005

   Plasmid

   #171632

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDA_763

   Plasmid

   #201156

   Purpose

   EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' NLS for CRISPRa

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   5x-ALFA tag-dEnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D908A

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs_FLAG-NMNAT1

   Plasmid

   #133259

   Purpose

   Express FLAG-tagged NMNAT1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NMNAT1 (NMNAT1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCRISPR v2-sgLKB1 clone2

   Plasmid

   #162126

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP1354

   Plasmid

   #119275

   Purpose

   for cloning new sgRNAs

   Depositor

   Jason Peters, Carol Gross, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGCP123-EbpA_g1

   Plasmid

   #153517

   Purpose

   sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. G1333 DddAtox-C–dSpCas9

   Plasmid

   #157834

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. NSP3-FR-2Strep

   Plasmid

   #210315

   Purpose

   Expresses SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   NSP3 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBHM2329

   Plasmid

   #171686

   Purpose

   pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   PCnU6-sgRNA_scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   C. neoformans U6

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. FgH1tUTG_mup53_Ex4

    Plasmid

    #85534

    Purpose

    Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    mu p53
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    H1t

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    E174R, R951K, R955A

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFDH

    Plasmid

    #131706

    Purpose

    Express constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coli

    Depositor

    Ron Milo

    Article

    Gleizer et al Cell. 2019 Nov 27;179(6):1255-1263.e12.

    Insert

    formate dehydrogenase
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLyGo-Ec-8

    Plasmid

    #163136

    Purpose

    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P(rhaB)

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRedCm

    Plasmid

    #176225

    Purpose

    LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB and PL

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-T7-NlaCas3-NLS-6xHis

    Plasmid

    #180214

    Purpose

    Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas3c
    

    Use

    Tags

    6xHis and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetroX-Tight-MCS_PGK-GpNLuc

    Plasmid

    #70185

    Purpose

    Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects..

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-FLAG-Neuroserpin

    Plasmid

    #58261

    Purpose

    Retroviral vector for expressing Neuroserpin in mammalian cells

    Depositor

    Joan Massague

    Article

    Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

    Insert

    Neuroserpin (SERPINI1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. LentiCRISPR v2-sgLKB1 clone1

    Plasmid

    #162125

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRetroX-Tight-MCS_PGK-OgNLuc

    Plasmid

    #70186

    Purpose

    Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. REEP6-EGFP

    Plasmid

    #210364

    Purpose

    Expresses REEP6 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    REEP6 (REEP6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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