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pLVX-EF1a-tetOn-IRES-G418 (EtO)

Plasmid

#84776

Purpose

Second generation lenti tetracycline operator (on)

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

TET OPERATOR

Use

Lentiviral

Promoter

pTIGHT

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 S15A

Plasmid

#69004

Purpose

mammalian expression of human p53 S15A mutant.

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Mutation

S15A

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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HPRT

Plasmid

#22884

Depositor

James Thomson

Article

Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Insert

hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)

Use

Cre/Lox; Homologous recombination

Mutation

PGK, NeoR, and BGH polyA are between the two geno…

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only

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1198 pAdTrack FKHR

Plasmid

#9036

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR (FOXO1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pSerOTS-C1* (V70)

Plasmid

#188537

Purpose

Phosphoserine incorporation into recombinant proteins

Depositor

Jesse Rinehart

Article

Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

Inserts

pSerRS
Ef-pSer
tRNApSer

Expression

Bacterial

Mutation

mutations resulting from laboratory evolution

Promoter

glnS*

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDY0750 pCAG-NLS-BceINT

Plasmid

#179112

Purpose

BceINT expression vector

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCAG-NLS-BceINT

Use

CRISPR

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV2.5-THP-dsRed2

Plasmid

#80335

Purpose

Fluorescent Reporter for Dopaminergic Neuron Differentiation

Depositor

Kwang-Soo Kim

Article

Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

Insert

rat TH promoter (Th Rat)

Use

AAV

Promoter

rat Tyrosine hydroxylase

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIalpha-T286A

Plasmid

#21220

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CAMKIIalpha-T286A (Camk2a Rat)

Tags

GFP

Expression

Mammalian

Mutation

T286A (Lab plasmid KS 0003).

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
V5-GFP-TurboID-KDEL_pLX208

Plasmid

#202020

Purpose

expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-TurboID-KDEL

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mRuby3

Plasmid

#155221

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)

Plasmid

#84777

Purpose

Second generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neurons

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

Ngn2 and Mash1 with a T2A (NEUROG2, ASCL1 Human)

Use

Lentiviral

Promoter

pTIGHT

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pUB_smHA_KDM5B_MS2

Plasmid

#81085

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smHA_KDM5B_24xMS2

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IN

Plasmid

#60612

Purpose

Backbone for tet-inducible genes

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM60-Tia1

Plasmid

#38243

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Tia1 (Tia1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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GST-PRMT6

Plasmid

#34700

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2002 Feb 1;277(5):3537-43.

Insert

PMRT6

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pBID

Plasmid

#35190

Depositor

Brian McCabe

Article

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Type

Empty backbone

Use

Drosophila transgene expression

Promoter

UAS

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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pAC149-pCR8-dCas9VP160

Plasmid

#48221

Purpose

dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR and Gateway Cloning

Tags

HA Tag and VP160

Mutation

D10A;H840A nuclease-deficient

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

Plasmid

#40810

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(31-51), S218E, S222D

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
pU6-PspCas13b-gRNA-Actb1216

Plasmid

#155368

Purpose

PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

PspCas13b gRNA targeting Actb 1216

Use

CRISPR

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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