We narrowed to 11,027 results for: ENA;

1. pAAV_SynP_DIO_tAKARα _WPRE

   Plasmid

   #196675

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   tAKARα
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-nuclear-EKAR4

   Plasmid

   #174439

   Purpose

   Nuclear-targeted EKAR4.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   nuclear-EKAR4
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. p15_attB(bxb)_lox

   Plasmid

   #183764

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

   Plasmid

   #116885

   Purpose

   FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RVH)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKDsgRNA-rssA

   Plasmid

   #89957

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   rssA gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

   Plasmid

   #117910

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R44A on eDHFR

   Promoter

   T7

   Available Since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDIRECT_25F

   Plasmid

   #91143

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEY14

   Plasmid

   #191059

   Purpose

   acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   acr-5(orf) (acr-5 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLGR002 LGR Cas9 guide vector

   Plasmid

   #188320

   Purpose

   Universal backbone for CRISPR/Cas9 sgRNA expression

   Depositor

   Laralynne Przybyla

   Article

   Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   PuroR-T2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   pU6 5'CMV and EF1-alpha

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDB_161

    Plasmid

    #216091

    Purpose

    Cas9 [Sp] CRISPRi targeting CD81, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD81 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat

    Plasmid

    #178883

    Purpose

    Vector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    U6-Nla repeat-BbsI-Nla repeat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

    Plasmid

    #116884

    Purpose

    FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

    Depositor

    Brian Kuhlman

    Article

    Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

    Insert

    LINXa4(RLR)-Bre1
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

    Promoter

    ADH1

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRDB_156

    Plasmid

    #216090

    Purpose

    Cas9 [Sp] CRISPRa targeting CD274, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD274 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. V5-LOV-Turbo-NLS

    Plasmid

    #199663

    Purpose

    expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NLS and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMP1183

    Plasmid

    #119254

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP1237

    Plasmid

    #119262

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBHM2404

    Plasmid

    #173441

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-NAT1
    

    Use

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-TCF4

    Plasmid

    #109144

    Purpose

    For the expression of GFP-TCF in mammalian cells

    Depositor

    Mariann Bienz

    Article

    Townsley et al Nat Cell Biol. 2004 Jul;6(7):626-33. doi

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

    Plasmid

    #190162

    Purpose

    Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

    Insert

    mcherry_(3'UTR, No Actin)_24Broccoli
    

    Use

    Tags

    24×Broccoli

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETDuet RagA-RagC

    Plasmid

    #99641

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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