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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,988 results for: LEA

Showing: 7851 - 7900 of 19988 results

  2. pSCL.039

    Plasmid
    #184973
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
    Use
    Tags
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184973-plasmid-map-sequence-id-376529

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  4. pNHT7

    Plasmid
    #186753
    Purpose
    A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.
    Depositor
    Bo Wang
    Article
    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002
    Type
    Empty backbone
    Use
    Synthetic Biology; Cloning backbone
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available Since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186753-plasmid-map-sequence-id-375095

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  21. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid
    #181748
    Purpose
    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Type
    Empty backbone
    Use
    Substrate plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181748-plasmid-map-sequence-id-354677

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  24. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161955-plasmid-map-sequence-id-326361

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  31. pET21-pelB-LaG-3

    Plasmid
    #172740
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-3
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172740-plasmid-map-sequence-id-340191

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  40. pET28a(+)-HisTim12_CtoA

    Plasmid
    #170281
    Purpose
    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2
    Insert
    yeast Tim12 (TIM12 Budding Yeast)
    Use
    Tags
    TEV-cleavable N-terminal His tag (on backbone)
    Expression
    Bacterial
    Mutation
    mutated two non-conserved Cys to Ala
    Promoter
    Available Since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170281-plasmid-map-sequence-id-334955

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  41. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161942-plasmid-map-sequence-id-329042

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  42. IBMc226

    Plasmid
    #161944
    Purpose
    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161944-plasmid-map-sequence-id-326341

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Showing: 7851 - 7900 of 19988 results