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dCAS9-VP64_GFP

Plasmid

#61422

Purpose

Expresses dCAS9-VP64 activator with 2A GFP

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Concentrated Lentiviral Prep

Insert

dCAS9(D10A,H840A)-VP64_2A_GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH NICD1

Plasmid

#86500

Purpose

Mammalian expression of Myc tagged NICD1

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

Cytoplasmic domain only (AA 1753-2531)

Promoter

EF1a

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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hCas9-VPR

Plasmid

#68497

Purpose

nuclease competent SP-Cas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SP-Cas9-VPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSN16

Plasmid

#100103

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Klotho (secreted)

Plasmid

#17713

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Tags

His and V5

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pENTR-c-Met

Plasmid

#31786

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Use

Entry vector

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2-UTX-F

Plasmid

#17438

Purpose

Expression of UTX in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pLCN DSB Repair Reporter (DRR)

Plasmid

#98895

Purpose

DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-ISce1-ISce1-eGFP

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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1099 pcDNA GFP FKHR AAA

Plasmid

#9023

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR AAA (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag hHMGB1

Plasmid

#31609

Depositor

Yasuhiko Kawakami

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

Insert

HMGB1 (HMGB1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pK170.AAV-TRE-Cre-WPRE (Supernova)

Plasmid

#85040

Purpose

For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nlsCre-WPRE

Use

AAV

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-ATG5

Plasmid

#99573

Purpose

Expresses Cas9 with sgRNA targeting Exon 7 of ATG5

Depositor

Edward Campbell

Article

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting ATG5 (ATG5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-NICD1

Plasmid

#46047

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Tags

Myc tag

Expression

Mammalian

Mutation

Cytoplasmic domain initiating at amino acid 1753

Promoter

EF1a

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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pBS1C

Plasmid

#55168

Purpose

Empty vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pMIEG3-JunDN

Plasmid

#40350

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

Jun dominant negative (JUN Human)

Use

Retroviral

Tags

HA and IRES eGFP

Expression

Mammalian

Mutation

first 122 amino acids deleted

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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Raf-1 GST RBD 1-149

Plasmid

#13338

Depositor

Channing Der

Article

Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-1

Insert

Raf-1 (RAF1 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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FUGW-eGFP-ZEB1 3'UTR

Plasmid

#81018

Purpose

FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

Zeb1 3'UTR (UTS2R Human)

Use

Lentiviral

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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Pveg

Plasmid

#55173

Purpose

Very strong constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

veg promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

veg

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 WT

Plasmid

#12301

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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CMV500 A-CREB

Plasmid

#33371

Depositor

Charles Vinson

Article

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

CREB (Creb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available Since

Dec. 19, 2011

Availability

Academic Institutions and Nonprofits only

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