We narrowed to 24,341 results for: Spr

1. pFYF1548 EMX1

   Plasmid

   #47508

   Purpose

   human gRNA expression vector targeting EMX1

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EMX1 (EMX1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. dpYPQ230-D156R-ABE5

   Plasmid

   #195371

   Purpose

   dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   ecTadA8e-(GGGGS)x8-dLbCas12a-D156R
   

   Use

   CRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…

   Tags

   5' and 3' NLS

   Expression

   Plant

   Mutation

   Promoter

   ZmUBI1

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pST1374-N-NLS-flag-linker-Cas9-H840A

   Plasmid

   #51129

   Purpose

   Cas9 nickase optimized for nuclear import.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Insert

   Cas9 nickase
   

   Use

   CRISPR

   Tags

   Flag and NLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAS9id

   Plasmid

   #141251

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SQT1665

   Plasmid

   #78744

   Purpose

   Human expression plasmid for LbCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere

   Plasmid

   #85715

   Purpose

   Telomere-targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   telomere sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS9cd

   Plasmid

   #141252

   Purpose

   Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ-dpcoCas9-SunTag

   Plasmid

   #158410

   Purpose

   CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. SQT1659

   Plasmid

   #78743

   Purpose

   Human expression plasmid for AsCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS9c

    Plasmid

    #141250

    Purpose

    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

    Plasmid

    #164038

    Purpose

    Expression of dCas9-14xGFP11 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    dCas9-14xGFP11
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    dSV40

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEL031

    Plasmid

    #137900

    Purpose

    STU2.0 SpyCas9 tRNA system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330A_D10A-1x5

    Plasmid

    #58775

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGal-BE3

    Plasmid

    #172409

    Purpose

    Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

    Depositor

    Leonid Kruglyak

    Article

    Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

    Insert

    BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GALL

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

    Plasmid

    #167902

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEL032

    Plasmid

    #137901

    Purpose

    STU2.0 LbCas12a for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. dpYPQ230-D156R-ABE8

    Plasmid

    #195569

    Purpose

    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    ecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible ectada8e-(gsagsaagsgef…

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    ZmUBI1

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-EF1adCas9VP64T2ABsd-W

    Plasmid

    #112919

    Purpose

    Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9VP64-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-T3-CjCAS9-pA

    Plasmid

    #131543

    Purpose

    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    FLAG-SV40NLS, SV40NLS, and polyA

    Expression

    Mammalian

    Mutation

    Human codon-optimized

    Promoter

    CAG, T3

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAcrVIB1

    Plasmid

    #184566

    Purpose

    Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    anti-CRISPR protein inhibiting Cas13b nucleases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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