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We narrowed to 11,938 results for: emb

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Showing: 3161 - 3180 of 11938 results

  2. BcLOV4 fusion tools screening plasmid #1

    Plasmid
    #174511
    Purpose
    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Publication
    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    174511-plasmid-map-sequence-id-357205

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  3. ORAI1-YFP L273D-L276D

    Plasmid
    #114182
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.
    Depositor
    Nicolas Demaurex
    Publication
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ORAI1-YFP L273D-L276D (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    L273D/L276D
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114182-plasmid-map-sequence-id-218681

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  4. mEos3-caRac1

    Plasmid
    #92041
    Purpose
    Expresses mEos3 fusion with caRac1 (Q61L)
    Depositor
    Ji Yu
    Publication
    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAC1 (RAC1 Human)
    Tags
    mEos3
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Q61L (constitutively active)
    Promoter
    CMV
    Available since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92041-plasmid-map-sequence-id-180751

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  5. pG4-E6s-luc

    Plasmid
    #46324
    Depositor
    Steven Brown
    Publication
    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5xGal4
    Use
    Luciferase
    Tags
    E boxes and luciferase
    Expression
    Mammalian
    Mutation
    five copies of a multimerized GAL4 (5×GAL4) site …
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pmCherryC1 VAP-A KD/MD

    Plasmid
    #226408
    Purpose
    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to mCherry in mammalian cells
    Depositor
    Catherine Tomasetto
    Publication
    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAP-A (VAPA Human)
    Tags
    T7-Xpress tag and mCherry
    Expression
    Mammalian
    Mutation
    K94D/M96D mutant, unable to bind FFAT motifs
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    226408-plasmid-map-sequence-id-447854

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  8. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184248-plasmid-map-sequence-id-370322

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  11. YFP-DAXX-mSIM1/2

    Plasmid
    #134525
    Purpose
    Expresses YFP-DAXX fusion protein with a mutations in both its 5' and 3' SIMs
    Depositor
    Susan Janicki
    Publication
    Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DAXX-mSIM1/2
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    SIM (IIVL) 7-10 aa changed to IGAG and SIM (IIVL)…
    Promoter
    CMV
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    134525-plasmid-map-sequence-id-264206

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  12. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

    Plasmid
    #102421
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Publication
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 (Tet1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Modified at the Dharmacon SMARTpool siRNA #2 targ…
    Promoter
    TetO-CMV
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102421-plasmid-map-sequence-id-198790

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  19. AP-APP

    Plasmid
    #196706
    Purpose
    Expresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cells
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Tags
    AP, Avitag and HA
    Expression
    Mammalian
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196706-plasmid-map-sequence-id-386447

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  20. ATX3-77Q

    Plasmid
    #184249
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Codon optimization for protein expression in BL2…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184249-plasmid-map-sequence-id-370367

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Showing: 3161 - 3180 of 11938 results