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Plasmid#109020Purposestable lentiviral expression of cDNADepositorInsertATL2 (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.N1-HDAC6.DC (catalytic deficient)
Plasmid#36189DepositorInsertHDAC6.DC (HDAC6 Human)
UseTagsEGFPExpressionMammalianMutationH216/611APromoterCMVAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
PERK 4: PERKKD-pGEX4T-1
Plasmid#21817DepositorInsertPERK (537-1114) (Eif2ak3 Mouse)
UseTagsGST and Myc (9E10)ExpressionBacterialMutationPERK Kinase Domain. Amino acids 537-1114.PromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F2
Plasmid#184164PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP-GFP-shch-TOG
Plasmid#69113PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP∆682-688-shTACC3
Plasmid#69116PurposeDual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationDeletion of amino acids 682-688 (region containin…PromoterCMVAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
PERK 5: PERK.K618R.pGEX4T-1
Plasmid#21818DepositorInsertPERK (537-1114) K618R (Eif2ak3 Mouse)
UseTagsGST and Myc (9E10)ExpressionBacterialMutationPERK Kinase Domain. Amino acids 537-1114. Kinase …PromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-Synectin
Plasmid#35791DepositorInsertSynectin (Gipc1 Mouse)
UseTagsEYFPExpressionMammalianMutationK111RPromoterCMVAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-CFP-STM2(15-746)
Plasmid#18863DepositorInsertStromal Interaction Molecule 2 (STIM2 Human)
UseTagsCFPExpressionMammalianMutationCFP inserted between signal peptide and residues …PromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMax-Synectin
Plasmid#35943DepositorInsertSynectin (Gipc1 Mouse)
UseTags6xHIS and XpressExpressionMammalianMutationK111RPromoterCMVAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHisAlix deltaPRD
Plasmid#42577DepositorInsertdelta-PRD ALIX (residues 1-702) (PDCD6IP Human)
UseTagsHIS-TEVExpressionBacterialMutationsilent mutation A1617G (numbering in ALIX gene)PromoterAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
dora[B]_rescue_construct
Plasmid#190637PurposePuromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[A]_rescue_construct
Plasmid#190639PurposePuromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-human OCRL
Plasmid#22207DepositorInsertOculocerebrorenal syndrome of Lowe (OCRL Human)
UseRetroviralTagsHAExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one
Plasmid#183695PurposeExpresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selectiveDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B2
Plasmid#172843PurposeCRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mRuby3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TPST1-EGFP
Plasmid#66617PurposeEncodes GFP fusion of TPST1 trans Golgi marker; (Tyrosyl protein sulfotransferase 2)DepositorInsertTPST1 (TPST1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ING2
Plasmid#109502PurposeMuscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsertactc1b-mKate2-ING2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
UseTagsExpressionWormMutationPromotereft-3Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_CHO-Bip_gRNA1
Plasmid#183694PurposeCRISPRa gRNA expression vector for Chinese Hamster Bip (Hspa5)DepositorInsertgRNA targeting chinese hamster Bip (Hspa5)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag_MmPERK_S503_N1114_pBabePuro
Plasmid#58423Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.DepositorInsertPERK (Eif2ak3 Mouse)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationDeleted amino acids M1 to Y502PromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC154-dual-dCas9VP160-sgExpression
Plasmid#48240PurposeDual expression construct expressing both dCas9VP160 and sgRNA from separate promotersDepositorInsertdCas9
UseCRISPRTagsHA-Tag, HA-tag, and VP160ExpressionMammalianMutationD10A H840APromoterAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-GFP-FCHo2
Plasmid#170870PurposeMammalian expresssion of His-GFP-FCHo2.DepositorInsertFCH domain only 2 (Fcho2) (Fcho2 Mouse)
UseTags(His)6 and mEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC152-dual-dCas9VP64-sgExpression
Plasmid#48238PurposeDual expression construct expressing both dCas9VP64 and sgRNA from separate promotersDepositorInsertdCas9
UseCRISPRTagsHA Tag, HA-Tag, and VP64ExpressionMammalianMutationD10A;H840APromoterAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔRING-Box
Plasmid#105517PurposeExpression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
UseTagsmCherryExpressionMammalianMutationdeletion of RING domain (aa124-462)PromoterCMVAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔPRYSPRY
Plasmid#105518PurposeExpression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
UseTagsmCherryExpressionMammalianMutationdeletion of PRYSPRY domain (aa1-281)PromoterCMVAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-Map4
Plasmid#105571PurposeT7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNADepositorInsertMAP4 (Map4 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SEP-Glur2 (MCA10)
Plasmid#64941Purposemammalian expression of rat GluR2DepositorInsertGluR2 (Gria2 Rat)
UseTagsSEP (superecliptic pHluorin)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC93-pmax-dCas9VP160
Plasmid#48225PurposedCas9VP160 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPRTagsHA Tag and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔPRYSPRY
Plasmid#105524PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-YFP-STM2(15-746)
Plasmid#18862DepositorInsertStromal interaction molecule 2 (STIM2 Human)
UseTagsYFPExpressionMammalianMutationYFP inserted between signal peptide(SP) of STIM1 …PromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAC153-dual-dCas9VP96-sgExpression
Plasmid#48239PurposeDual expression construct expressing both dCas9VP96 and sgRNA from separate promotersDepositorInsertdCas9
UseCRISPRTagsHA-Tag, HA-tag, and VP96ExpressionMammalianMutationD10A H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.N1-HDAC6.delta Buz
Plasmid#36190DepositorInsertHDAC6..delta Buz (HDAC6 Human)
UseTagsEGFPExpressionMammalianMutationDeletion of the C-terminal ub binding domain (BUZ…PromoterCMVAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAC91-pmax-dCas9VP64
Plasmid#48223PurposedCas9VP64 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPRTagsHA Tag and VP64ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔRING-Box
Plasmid#105523PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationdeletion of RING domain (aa124-462)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC84-pCR8-dCas9
Plasmid#48218PurposedCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A)
UseCRISPR; Gateway donorTagsHA TagExpressionMutationD10A;H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21ΔRING-Box
Plasmid#105520PurposeT7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationdeletion of RING domain (aa124-462)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 COLIA1 full length 3'UTR
Plasmid#26993DepositorInsertCOL1A1 Full length 3' UTR (COL1A1 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2-R244Q
Plasmid#109023Purposestable lentiviral expression of cDNADepositorInsertATL2-R244Q (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationArg244Gln mutation results in dimerization defectPromoterEIF-1a promoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC92-pmax-dCas9VP96
Plasmid#48224PurposedCas9VP96 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP96 activation domain
UseCRISPRTagsHA Tag and VP96ExpressionMammalianMutationD10A;H840APromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21ΔPRYSPRY
Plasmid#105521PurposeT7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 MERTK full length 3'UTR
Plasmid#26992DepositorInsertMERTK Full length 3' UTR (MERTK Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one_Bip gRNA1
Plasmid#183696PurposeExpresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selectiveDepositorInsertgRNA targeting chinese hamster Bip (Hspa5)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[B]_rescue_construct
Plasmid#190638PurposePuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2-deltaTM
Plasmid#109021Purposestable lentiviral expression of cDNADepositorInsertATL2-deltaTM (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-P1Egfp
Plasmid#90147Purposecontains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4E2P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL2-1
Plasmid#109008PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL2-sgRNA #1 (ATL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc
Plasmid#138368PurposeLentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoterDepositorInsert161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-basEgfp
Plasmid#90144Purposecontains dll4 enhancer 2 sequence and a basal promoter driving expression of eGFPDepositorInsertdll4E2basEgfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only