We narrowed to 344 results for: IPTG
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Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TnY
Plasmid#163975PurposeIPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.DepositorInsertTnY
TagsMxe intein - Chitin-binding domainExpressionBacterialAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHT-6xR.ads-PxylA-DI
Plasmid#47386PurposeExpresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsads
dxs
idi
Tags6xR and HisExpressionBacterialPromoterPgrac and PxylAAvailable SinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Q58L wgMDH
Plasmid#204246PurposeBacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationQ58LPromoterT5Available SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-cTF
Plasmid#87743PurposeIPTG-inducible expression of ligase-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and cTF (chimeric transcription factor base…ExpressionBacterialPromoterT5-lacAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEC3111 (pSpy1C-Plac(3)_ffluc)
Plasmid#218778Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(3)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY118A
Plasmid#182958PurposepSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.DepositorInsertsCas9
Gam, Beta, Exo
I-scel
Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_AB
Plasmid#66004PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-pprA
Plasmid#87744PurposeIPTG-inducible expression of ligase-pprA fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and codon optimized pprA (from Deinococcus …ExpressionBacterialPromoterT5-lacAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121A wgMDH
Plasmid#204252PurposeBacterial expression of G121A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG121AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123A wgMDH
Plasmid#204254PurposeBacterial expression of P123A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationP123AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127F wgMDH
Plasmid#204264PurposeBacterial expression of G127F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG127FAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126R wgMDH
Plasmid#204261PurposeBacterial expression of P126R wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationP126RAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127A wgMDH
Plasmid#204263PurposeBacterial expression of G127A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG127AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127P wgMDH
Plasmid#204265PurposeBacterial expression of G127P wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG127PAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121F wgMDH
Plasmid#204253PurposeBacterial expression of G121F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG121FAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126G wgMDH
Plasmid#204260PurposeBacterial expression of P126G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationP126GAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123G wgMDH
Plasmid#204256PurposeBacterial expression of P123G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationP123GAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123F wgMDH
Plasmid#204255PurposeBacterial expression of P123F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationP123FAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPodA11
Plasmid#162843PurposeExpression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistanceDepositorInsertProtein Designed Pyocyanin demethylase
TagsTEV-cleavable 6x-His tagExpressionBacterialMutationA53N, I73T, A87V, M99V, A129T from WT PodAPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only