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We narrowed to 10,854 results for: kars;

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pLKO.1P ERCC2_1

Plasmid

#160781

Purpose

Suppress ERCC2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shERCC2_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P DNA2_2

Plasmid

#160780

Purpose

Suppress DNA2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shDNA2_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P DNA2_1

Plasmid

#160779

Purpose

Suppress DNA2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shDNA2_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P CISD1_2

Plasmid

#160774

Purpose

Suppress CISD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCISD1_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P BRIP1_2

Plasmid

#160772

Purpose

Suppress BRIP1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shBRIP1_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P BRIP1_1

Plasmid

#160771

Purpose

Suppress BRIP1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shBRIP1_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25C_2

Plasmid

#160770

Purpose

Suppress CDC25C

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25C_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25C_1

Plasmid

#160769

Purpose

Suppress CDC25C

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25C_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25A_1

Plasmid

#160767

Purpose

Suppress CDC25A

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25A_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJT140_GalL_cCDA1-VRERBE3

Plasmid

#145103

Purpose

Expressing base editor cCDA1-VRERBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-VRERBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

Promoter

GalL

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT176_GalL_eA3A-BE3

Plasmid

#145125

Purpose

Expressing base editor eA3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(N57G)-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

A3A(N57G); spCas9(D10A)

Promoter

GalL

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT174_GalL_A3A-Y130F-BE3

Plasmid

#145123

Purpose

Expressing base editor A3A-Y130F-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(Y130F)-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

A3A(Y130F); spCas9(D10A)

Promoter

GalL

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT173_GalL_A3A-R128A-BE3

Plasmid

#145122

Purpose

Expressing base editor A3A-R128A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(R128A)-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

A3A(R128A); spCas9(D10A)

Promoter

GalL

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT122_GalL_cCDA1-VQRBE3

Plasmid

#145087

Purpose

Expressing base editor cCDA1-VQRBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-VQRBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A, D1135V, R1335Q, T1337R)

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT113_GalL_cCDA1-xBE3

Plasmid

#145079

Purpose

Expressing base editor cCDA1-xBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-xBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT2

Plasmid

#127512

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 2.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 2 coding sequence codon optimized for H. sapiens expression.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT4

Plasmid

#127513

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 4.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 4 coding sequence codon optimized for H. sapiens expression.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT5

Plasmid

#127514

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 5.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 5 coding sequence codon optimized for H. sapiens expression.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT9

Plasmid

#127516

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 9.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 9 coding sequence codon optimized for H. sapiens expression.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT13

Plasmid

#127517

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 13.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 13 coding sequence codon optimized for H. sapiens expression.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-R1597W-VWF

Plasmid

#124826

Purpose

Expresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and R1597W mutation A2 domain (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-D1498A-VWF

Plasmid

#124825

Purpose

Expresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and D1498A mutation A2 domain (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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PME-1_R369D mutant

Plasmid

#119294

Purpose

Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the PP2Ac binding motif

Depositor

Jukka Westermarck

Article

Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

Insert

PPME1 (PPME1 Human)

Use

Tags

Expression

Mammalian

Mutation

R369D

Promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-HSF4b K294R

Plasmid

#118359

Purpose

This plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Use

Tags

Expression

Mammalian

Mutation

K294R

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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PAK4-FL-3'UTR-MT2

Plasmid

#113093

Purpose

Luciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutated

Depositor

James Donahue

Article

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Second predicted miR-199a-3p binding site mutated…

Promoter

PGK

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PAK4-FL-3'UTR-MT-both

Plasmid

#113094

Purpose

Luciferase reporter containing PAK4-FL- 3' UTR with both the predicted MiR-199a-3p binding sites mutated

Depositor

James Donahue

Article

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Both the predicted miR-199a-3p binding site muta…

Promoter

PGK

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-owlTRIMCyp-FOS K283D Q287D

Plasmid

#79068

Purpose

Express owl monkey TRIMCyp K283D, Q287D in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey) (K283D, Q287D)-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

CMV

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCIG1_UL136_26kDa_Myc

Plasmid

#74955

Purpose

Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Insert

UL136 (UL136 Human Cytomegalovirus strain TB40/E)

Use

Lentiviral

Tags

Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)

Expression

Mammalian

Mutation

delta (1-62) M78A M80A M100A M128A

Promoter

CMV, Lentiviral LTR

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG1_UL136_33kda_Myc

Plasmid

#74957

Purpose

Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Insert

UL136 (UL136 Human Cytomegalovirus strain TB40/E)

Use

Lentiviral

Tags

Myc tag (EQKLISEEDL)

Expression

Mammalian

Mutation

M63A M78A M80A M100A M128A

Promoter

CMV, Lentiviral LTR

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1

Plasmid

#42185

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Gamma 3(F70A, F71A) (Gng3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

F70A, F71A

Promoter

cmv

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only