We narrowed to 55,127 results for: NAM

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pSUM36-MCS2-myc

Plasmid

#109379

Purpose

Shuttle vector for high level expression in mycobacteria.

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

MCS2-Myc

Tags

Myc

Expression

Bacterial

Promoter

Kanamycin

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-sfGFP-kanMX (pMaM17)

Plasmid

#173452

Purpose

Contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

Insert

mCherry-sfGFP-ADH1ter

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MYC-Sumo3 WT

Plasmid

#48964

Purpose

Expresses MYC-Sumo3 Wild Type in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO3 (SUMO3 Human)

Tags

MYC (EQKLISEED)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-OTp-GCaMP6s

Plasmid

#192945

Purpose

To drive GCaMP6s under the control of mouse oxytocin promoter

Depositor

Kazunari Miyamichi

Article

Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

Insert

GCaMP6s

Use

AAV

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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TrkB Acceptor

Plasmid

#83953

Purpose

FRET acceptor for TrkB mEGFP

Depositor

Ryohei Yasuda

Article

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Phospholipase C gamma 1 (Plcg1 Mouse)

Tags

mRFP

Expression

Mammalian

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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#1.4 pre-R-SynapShot-TagBFP

Plasmid

#214719

Purpose

Expresses red #1.4 pre-SynapShot and TagBFP

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 pre-R-SynapShot

Use

AAV

Tags

TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mCardinal-P2A-GFP

Plasmid

#197167

Purpose

Expresses the protein of mCardinal-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mCardinal-P2A-GFP

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209724

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.HaloTag

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher002

Plasmid

#102829

Purpose

Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Cys-SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Cysteine residue in N-terminal region

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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FUGW-d2GFP-200

Plasmid

#79602

Purpose

FUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sites

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

d2GFP + miR-200 family targets

Use

Lentiviral

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-PAK2 binding domain

Plasmid

#83951

Purpose

FLIM acceptor for mEGFP Rac1

Depositor

Ryohei Yasuda

Article

Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

Insert

Pak2 (PAK2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

aa60-113 only; S74A F84A

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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VinculinTS-A50I/I997A

Plasmid

#111829

Purpose

Vinculin tension sensor with point mutations affecting ability to bind talin and actin

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A)

Expression

Mammalian

Mutation

changed vinculin alanine 50 to isoleucine and iso…

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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Dsup pLVX-Puro

Plasmid

#180635

Purpose

Expresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio tolerance

Depositor

Christopher Mason

Article

Westover et al bioRxiv 2020.11.10.373571

Insert

Dsup

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 5'-myr-dEGFP-3'

Plasmid

#16075

Depositor

Erin Schuman

Article

Aakalu et al Neuron. 2001 May . 30(2):489-502.

Insert

Protein synthesis reporter (Camk2a Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pSGKP_AcsgRNA

Plasmid

#203807

Purpose

pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcgRNA

Use

CRISPR

Promoter

J23119

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV7 mitoDendra2

Plasmid

#157757

Purpose

Expression of the fluorescent protein Dendra2 targeted to mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

Dendra2

Expression

Mammalian

Mutation

MTS coxVIII +Dendra2

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTC217

Plasmid

#70018

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Expression

Plant

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBJ3

Plasmid

#167132

Purpose

A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Expression

Bacterial

Promoter

Frr promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA 3.1 Flag-rat-PTP1B

Plasmid

#35988

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pICH-nuc-roGFP2-ORP1

Plasmid

#191758

Purpose

Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

nuc-roGFP2-ORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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