We narrowed to 28,006 results for: met

1. PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

   Plasmid

   #110623

   Purpose

   It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   PH mScarlet-I- IRES- sYFP2 PH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. peCALWY-NB

   Plasmid

   #22239

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY non-binding variant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C284S, C287S, C413S and C416S mutations linker c…

   Promoter

   Available Since

   Jan. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSMVP-Cas9FL-P2A-turboGFP

   Plasmid

   #80941

   Purpose

   Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9FL
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrans_9xD3B_Cluc_Norm

   Plasmid

   #68415

   Purpose

   CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   Cypridina Luciferase-2A-Venus YFP
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   YFP: M153T,V163A,S175G ("Venus") YFP Va…

   Promoter

   minimalCMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ1663

   Plasmid

   #51484

   Purpose

   miniMos transposable element with HygroR and peel-1, MCS cloning

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas_5+7_gfp

   Plasmid

   #60595

   Purpose

   encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cas_5+7_gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas_2+5

   Plasmid

   #60594

   Purpose

   encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cas_2+5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ1655

   Plasmid

   #51481

   Purpose

   miniMos transposable element with HygroR, R4-R3 Gateway cloning

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. M-SP-sgRNA

   Plasmid

   #48671

   Purpose

   Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEB1-moxVenus

    Plasmid

    #103993

    Purpose

    Plasmid encoding moxVenus

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    moxVenus
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEmerald-Talin-N-10

    Plasmid

    #54266

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

    Insert

    Talin
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. mEmerald-Paxillin-22

    Plasmid

    #54219

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

    Insert

    Paxillin
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pEB1-mClover3

    Plasmid

    #103994

    Purpose

    Plasmid encoding mClover3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mClover3
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD/HisB-PhoCl-MBP

    Plasmid

    #87689

    Purpose

    Express PhoCl-MBP in E.coli

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl fused to Maltose-Binding Protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-OmpT

    Plasmid

    #68862

    Purpose

    construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

    Depositor

    Neil Kelleher

    Article

    Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

    Insert

    OmpT (ompT )
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-GoldenGate

    Plasmid

    #47388

    Purpose

    Expression plasmid for TALE proteins compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. XW08

    Plasmid

    #65832

    Purpose

    Punc-4-QF-SL2-mcherry-3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    OR74A quinic acid utilization activator (qa-1F)
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-4

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. TNXXL-CTS20

    Plasmid

    #48342

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    CTS20
    

    Use

    Tags

    TN-XXL

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pTriEx-mCherry-LOV2 V416I

    Plasmid

    #81045

    Purpose

    mammalian expression of LOV V416I

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV V416I
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    V416I

    Promoter

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-StrepII-TagRFP-CohI

    Plasmid

    #58710

    Purpose

    Detection construct to label dockerin type I containing fusion proteins

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    TagRFP-CohI
    

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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